Multi channel, dual chip dialysis device
A two multi-channel chip device was fabricated with gaskets and PDMS caps. The Upper fluidic
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I’ve been working recently with amyloid-beta (AB) provided to me by some people in Brad Nilsson’s lab. These experiments are related to the HMM diffusion experiments for our collaboration with Harold Smith. Basically, AB fibrils are very long (microns) and we are looking for ways to either prevent their aggregation from smaller peptides/oligos, or to…
Rhodamine concentration curve
Lingyun and I used the stir cell and spectrometer to measure the diffusion of rhodamine across the membrane. This was the first time I was able to construct an experiment in the stir cell while keeping both membranes intact. In the past one membrane would rupture or the cell would leak too much. For this…
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Separation of Microbeads from Whole Blood on 8 and 9 Micron Slit Membranes
ByKileanA few weeks ago, Josh and JP brought me some 3 window microslit membranes that had edge aligned 8 and 9 μm slits. While these may seem to be quite large for most applications, they are in fact perfect for working with whole blood. The experiments that we have planned for these membranes are based on the…
Gold separations with carbonized membranes
Recently, I have been trying to pass 5 nm, 10 nm, and 15 nm gold through untreated, carbonized, and ozone/carbonized membranes. These experiments were done in the pressure cell with fresh samples. I used a 1:1 dilution of Au stock and DI water. To wet the membrane I placed a 40 uL drop of water…
Bend.3 + Geltrex Tube Formation Studies
The following diagram lays out each separate setup that was tested using different combinations of Geltrex and substrates. Each trial has a different reasoning behind why it was done. The purpose of these trials was to investigate the effects of Gelrex gel and PCTE membrane inserts on tube formation in Bend.3 cells. First, we showed…