On Friday, Henry was kind enough to ship 15 tested Sepcons to the Barcikowski group in Essen, Germany. With luck they will arrive in time for Christmas. I wrote up some documentation and an illustration for wetting and using the Sepcons and figured I would share it with the group. Unfortunately, wordpress won’t let me upload the original illustrator .ai file, but here is a Word Document and below is a jpeg.
While imaging GFP tagged Staphylococcus aureus on our membranes within µSiM-CA devices, the question of whether or not we can differentiate between bacteria on the top and bottom of the membrane versus within the membrane has persisted. We know that during our experiments bacteria exist in all three of these places, therefore the question really is whether…
In order to test the sealing of the capillary tubing the PDMS I made a batch of PDMS with a recess in it (from a dime). Once this cured I used the UV Ozone process (@25C) to bond the PDMS to a glass microscope slide. When I arrived this morning the system was fully bound….
As the title states, I partially etched some different material (Wafer 2238, 100 nm NPN) to create nanofeatures with blown out holes. With a thicker membrane, we can blow out the pores more (etch longer) without compromising the strength of the material (with regard to templating purposes). These membranes were fractured and coated with 6…
Introduction Mural cells, including smooth muscle cells and pericytes, are important for supporting endothelial cells in the vasculature [1]. They play a role in angiogenesis, and contribute to the development of the vascular basement membrane that provides structure for blood vessels [1,2]. Numerous in vitro models of the blood-brain barrier (BBB) have incorporated pericytes alongside…
I am doing a few more membrane transfers and trying to troubleshoot my delamination problems, and I wanted to run my protocol by you guys before I mess up any more chips. I was able to get about 3/7 transfers visibly very good without any wrinkles, but they all still delaminate, either during the fabrication…
Submitted on March 14, 2014 Submitted Manuscript (PDF) Check Status
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the folks at Germany already got there stuff, damn these guys at DHL are fast.