MgF2 + Si3N4 Nanomembrane Images
Here are some images of deposited magnesium fluoride films on 50 nanonitride:
Here are some images of deposited magnesium fluoride films on 50 nanonitride:
I wanted to have 3 repeats of the previous salt-tuned separations. I’ve gone through and performed the densitometry on a couple more gels and compiled the results. 100mM: The first two experiments were from the two experiments set up on the same day. The third (yellow triangle) was set up a different day and does…
So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from the contact impedances and measure only the potential drop across the sample under consideration. See…
I would like to introduce Compressive Structured Illumination Microscope (CS-SIM), which will be built in the McGrath’s Lab. CS-SIM is a novel microscopy framework, which was proposed by me. It is dedicated to alleviate some SIM problems such as motion artifacts as well as photobleaching, and also it might present further resolution improvement thanks to…
One of Christine’s projects was to determine if pinholes affected electrical resistance (TEER) measurements. She summarized her work here. I collected her data and did some negative controls to finish this work up. Here’s the graph: The “no membranes” data is from Sepcon transwells with pnc-Si chips but without membranes (I broke them before assembling…
[latexpage] Last summer, Tejas and I spent a few days beating our heads against the wall because I was trying to develop a mathematical model of particle capture. Specifically, I was trying to understand what amount of transmembrane pressure we would need to hold a particle on (or in, as we are a politically correct,…
After some experimentation with acetylene concentration and anneal temperature, I was able to find a carbonization recipe that appears to shrink our pores by 1/3 the original diameter. Below are images of an untreated sample (TEM 148: 15 nm pnc-Si 1000 RTP) and a carbonized sample (see recipe at end of post). A closer look…