Silicon Ideal to Culture Patient-On-a-Chip | Insight & Intelligence™ | GEN

With the goniometer nearly finished, I’d like to share a look at what has been created: Introduction A surface contact angle goniometer (SCAG) measures the angle(s) of contact that a droplet of liquid makes with a surface. This particular SCAG does this by creating a shadow that is isometric with the droplet and recording its…

Last week Jess and I did some diffusion tests using the prototype chamber. We observed there was some fluorescence background noise from the plastic substrate. So we decided to make the buffer well (the hole on the substrate) all the way through the Polycarbonate sheet and seal the well with a slide. We can see…

I discussed with Jess the possibility of using a negatively charged dye. People did use such dyes before but we probably need to order them at this time. Since now we can use the spectrophotometer to determine the concentration of a solution, we might be able to make use of the potassium permanganate (KMnO4) solution…

I measured burst pressures on two non-unifrom wafers w391, w393. They have lots of pinholes (especially w391) but the pressure they withstand is pretty high. I expected it to be much lower after looking at the membranes in the microscope.

The last few times the spinned PDMS layers were much more uniform. The first picture below is with 1:100 diluted PDMS layer coated on it and the second one is a clean sample from the same wafer for comparison. The PDMS layer on the first picture is approximately 60nm thick. The measured thikness produced by…

Now that I’ve got plenty of fluorescent protein, I’ve moved back to testing protein adsorption to various surfaces. I’ve repeated the n-Dodecyl β-D-Maltoside (“DDM”) coating procedure (reference) with the goal of preventing or heavily decreasing the protein adsorption to the PDMS surface. This time I had a total of four samples: DDM treated, sham treated, untreated,…