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Update on ECM alignment mechanosensing and cell polarization using microporous membranes as masks
As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels. Here is the latest design: For these experiments we continued to use relatively low…
Parameterizing Nanovolcanoes
Having generated some tomograms of various Au, NPN, and MgF2 nanostructures in the past months, I should be able to parameterize these structures. I have many questions about how the nanostructures actually effect SERS performance. In general, we can use these methods to more accurately and precisely describe our nanomembranes. Hypothetical Factors Surface Roughness ~=…
Single-Well CytoVus w/ SiO2 Membrane – Comparisons
I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them. From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…
ITO Etching
Found a Master’s thesis from RIT that details etch rates for ITO films in Hydrochloric Acid. I originally attempted to do an etch using 1M HCl, but as the graph shows, this is not enough to produce an etch rate. I found this information out later, as 37% aqueous HCL = 12M solution, from http://openwetware.org/wiki/Hydrochloric_acid. Using…
Development of Arising Retinal Pigment Epithelium-19 (ARPE-19) Barrier Tissues on NPN
Author: Kevin Ling Dysfunction of the outer retinal blood barrier (oRBB), which is composed of the retinal pigment epithelium (RPE), Bruch’s membrane, and choriocapillaris (CC), is implicated in age-related macular degeneration (AMD), which causes irreversible central vision loss [1-4]. AMD is the leading cause of blindness in industrialized countries; it is estimated that by 2040,…
Electron Beam Lithography Microchannel Fabrication
This post is a continuation of project found in Fabricating 0.5 um Channels in Microscope Slides for Staph Invasion Assays. Just to review, after the development of μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019), Jim wanted to develop a platform to answer some more biophysics focused questions. In order…

