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Noncytotoxic dye SiR-DNA can be used for advanced automated tracking of cells
What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…
Oxygen RTP better than Argon RTP
In continuation of the previous post, its been further observed that O2 RTP treated samples remain unchanged whereas the Ar RTP treated samples started to discolor after a week, as shown in the following images: In order (left to right)1. Ozone+Ar RTP2. Ozone+O2 RTP3. Control (Untreated)4. Ar RTP5. O2 RTP
EO Update
ByJimOn the 13th of September we reviewed our recent progress on EO. Maryna and Jirachai (Borkholder group) gave presentations. I’ve uploaded PDF files of each of their presentations. Maryna’s presentation: MarynaEO Jarachai’s presentation: EO_Testing_Report (PPT) EO_Testing_Report (PDF) Bubble formation in electrolytic cells
Recent Discoloration in other Projects
Bernhard Group: Discoloration was observed during DNA separation experiments. Further study led to these results: No discoloration observed with low pH buffered NaCl. Discoloration with high pH buffered NaCl. Worried that Cl was the culprit, sodium acetate was tested. Discoloration observed with sodium acetate. In one early experiment, 4 areas were tested on one chip. …
Discoloration in Sepcon
We have seen that discoloration of chips is slower in Sepcons and this has been speculated to be because of the separation between the ‘well side’ and the ‘membrane side’ of the chip existing in the Sepcon format. To further provide evidence to this theory, I carried out an experiment monitoring the discoloration of samples…
MgF2 Nanomembrane Fabrication on partially etched NPN substrates
This work is a continuation of my last post, Partial Etch RIE Blows out NPN Pores, where I take the process a step further and deposit MgF2 onto the newly opened substrates. I used the same etch recipe to blow out the pores (0, 10, 20, 30, 40s) and then evaporated MgF2 onto these new substrates…

