Super useful conversions between volumes and lengths
- 1 m^3 = 1000 L
- 1 cm^3 = mL
- 1 mm^3 = uL
- 1 um^3 = fL
- 100 µm x 100 µm x 100 µm = 1 nL
- 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
Hi all, Today I would like to briefly mention the important parameters to consider when saturating a substrate with proteins by simple adsorption. More specifically, the concentration and time required to fully saturate a substrate ares estimated for systems with different geometry (see the PDF attachment): [NRG] surface adsoprtion The first-order approximations entailed in the above…
Introduction As discussed in a previous post, we are working to adapt a PDMS flow insert as a manufacturable component. While our priority was maintaining the original flow channel geometry as Mansouri et al. designed, we slightly changed the channel length (5 mm –> 4.6 mm) to accommodate an extra bonding area for the pressure-sensitive…
Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup. The flow module is a microfabricated PDMS insert that contains a channel over the membrane…
Wright, E., Miller, J.J, Csorda, M.. Gosselin, A.R., Carter, J.A., McGrath, J.L., Latulippe, D.R., Roussie, J.A. (2020) Development of Isoporous Microslit Silicon Nitride Membranes for Sterile Filtration Applications Biotechnology & Bioengineering https://doi.org/10.1002/bit.27240 DesOrmeaux, J. P., Winans, J. D., Wayson, S. E., Gaborski, T. R. , Khire, T. S., Striemer, C. C., and McGrath, J. L….
When we coat surfaces or particles with proteins we often wonder, ‘how long until the coating is done? ‘ In this 2009 paper we addressed the question directly. We soaked 100 nm polystyrene particles in 10% serum (about 6 mg/ml protein) for different periods of time, washed the particles to remove unbound protein, soluablized the…
Introduction The modular μSiM is based on a 96-well plate format with a 100 μl open well. While these devices have proven extremely useful for various barrier models including the blood-brain barrier1 and the tendon-vascular interface2, there is a need to transform the open well into a fluidic channel. A closed channel device would enable…