Super useful conversions between volumes and lengths
- 1 m^3 = 1000 L
- 1 cm^3 = mL
- 1 mm^3 = uL
- 1 um^3 = fL
- 100 µm x 100 µm x 100 µm = 1 nL
- 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1 aL
Wright, E., Miller, J.J, Csorda, M.. Gosselin, A.R., Carter, J.A., McGrath, J.L., Latulippe, D.R., Roussie, J.A. (2020) Development of Isoporous Microslit Silicon Nitride Membranes for Sterile Filtration Applications Biotechnology & Bioengineering https://doi.org/10.1002/bit.27240 DesOrmeaux, J. P., Winans, J. D., Wayson, S. E., Gaborski, T. R. , Khire, T. S., Striemer, C. C., and McGrath, J. L….
Knowledge of the particle number is critical in many of our applications. In trying to rationalize membrane ‘clogging’ by particles for example, it is wise to compare the particle density to pore density at the membrane. When conjugating protein to NPs, it is important to work in a molar excess of protein. In using nanoparticles…
Introduction As discussed in a previous post, we are working to adapt a PDMS flow insert as a manufacturable component. While our priority was maintaining the original flow channel geometry as Mansouri et al. designed, we slightly changed the channel length (5 mm –> 4.6 mm) to accommodate an extra bonding area for the pressure-sensitive…
One of the more useful formulas for our materials is Equation 1 in Chung et al., 2014 Lab on a Chip 14:2456-68 Assuming uniformly distributed pores in a planar (i.e. ultrathin) membrane, the relationship between pore radius r, porosity p, and the average spacing between pores, Lp-p is: Where the porosity is a number between…
This figure appears in our 2016 JMS paper and describes the clogging behavior of NPN with a 37 nm average pore size. The y-axis is the volume of filtrate after 1 min of forward centrifugation at 690g (3000 RPM). The x-axis is particle concentration in #/ml. The particles are polystyrene. Note we chose a size…
Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup. The flow module is a microfabricated PDMS insert that contains a channel over the membrane…