Quality System Update (June ’25)

The μSiM Vasc-SkM Microphysiological development can be separated into 4 parts: Media tuning for integrated culture Force sensing element setup Assembly of the μSiM Vasc-SkM and setting up the perfusion Transendothelial Electrical Resistance (TEER) measurement of the Vast component Work has been done on the first 2 parts. Media tuning for integrated culture In order…

Image analysis for force from videos of deflected PDMS beams In the summer, Yimei Du from University of Rochester visited Truskey lab to help with the μSiM Vasc-SkM system. Besides working with the chip culture, she helped improve the image analysis of the videos of PDMS beam deflection for more accurate myobundle force analysis. We…

We’ve long speculated that adding fluid flow over our tissue models would reduce the concentration of pro-inflammatory factors secreted in response to inflammatory challenges, dampening the overall inflammatory state compared to static culture. We now have evidence that this is true and the results are important as benchmarks for our future studies. In response to…

The following site may be useful to draw to people’s attention when specifying objectives on the Etaluma: https://www.microscope.healthcare.nikon.com/microtools/resolution-calculator/#Focus I have configured a custom resolution calculation using the sensor data supplied by Etaluma, here exemplified at 10x/0.3NA: To take account of the shorter tube lens (which gives ¼ magnification expected cf Nikon 200mm tube) I have…

Work in progress … **************** T cell–Engaging Immunotherapies Pose a Predictable but Complex RiskT cell–engaging bispecific antibodies (BsAbs), particularly those targeting CD3 and a tumor-associated antigen, have emerged as a powerful class of immunotherapies. These agents leverage endogenous T cells to induce targeted tumor cell death, often achieving deep and durable responses in hematologic malignancies….