R6G diffusion observed through the plastic substrate

Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in the incubator with untreated w638 chips as controls. All of the chips discolored within a…

Although the connection of those electrical circuits was always unstable, Greg and I did get some TEER measurement with this set up:   Device A Day 1 Day 2 Day 3 Day 4 Day 5 EVOM 1 -3450Ω* -1000Ω -1200 Ω -1000 Ω / EVOM2 100 Ω -9600 Ω 450 Ω -3 Ω 827Ω Device…

Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.

I’ve been using a Makerbot 2 for the last week or so, trying to see if we can use the same PDMS contact lithography techniques that we are all familiar with. In short, yes, but there is plenty of work to be done to make it a robust process for microfluidics.   Makerbot Printer The…

In attempting to generate control systems for our exosome work as well as to potentially develop a system that would help some collaborators at the University of Illinois, I have been working with the ultracentrifuge again to hopefully isolate a pure fraction of exosomes, devoid of any protein components. This would allow us to start…

Wafer 398 was deposited on 2/14/2008 but just etched yesterday in the new cell (it’s a beauty esp. when you compare it to the old one). I’ve etched several wafers now in the new cell and none of the membranes show signs of cigarette burns or tears. Good news in regards to the copper contamination…