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BMES 2018 Takeaways
ByAlecBMES – Atlanta 10/17/18-10/20/18 F-actin Alignment Quantification Using HSB Color Coded Maps ImageJ Plugin: OrientationJ Purpose: Quantification of F-actin alignment using ICC. HSB map provides a visual representation of fiber alignment with color codes related to hue:local orientation, saturation:coherency, brightness:fiber stain intensity (Figure 1). Our use: Quantification of HUVEC alignment before and after flow. Fluorescent…
PDMS-Membrane Bonding Update
ByMike HThis is just an update on the progress being made towards creating a closed flow cell by bonding PDMS to our membrane chips. For those who were not at the meeting on Tuesday we have successfully bonded PDMS to both glass cover slips as well as out membrane chips using the UV Ozone cleaner. Yesterday…
Introduction to the Membrane Flow Analysis Device for Gas (MFAD-G), QC Model
Introduction The Membrane Flow Analysis Device for Gas (MFAD-G), Quality Control Model, is the first* of a 3-version series of devices that, altogether, will allow use-specified measurement of flow rate and flow pressure of gas and liquid media through chip-mounted membranes. The QC Model has a rudimentary form of the general system and acts as…
The effects of driving pressure on sieving behavior in SiN Sepcons
We’ve been wondering for some time just how the thinness of our membrane will affect the concentration polarization behavior of solutes. Because solutes with sieving coefficients less than 1 are (by definition) moving slower than the solvent around them, in the area before the membrane their concentration increases. In the case of proteins, this so-called…
SLAS 2017 Conference Recap
SLAS – Society for Laboratory Automation and Screening https://www.slas.org Annual Conference February 6-8, 2017 in Washington DC Attended by Tom Gaborski for informative purposes. Collaborators/colleagues in attendance: Vinay Abhankar (UT-Arlington), Andrea Mazzocchi (alum @ Wake Forest), August Allen (alum @ Recursion Pharmaceuticals) My goal in attending this conference was to learn more about high content…
Two-step anneal trial
Last week I mentioned that a two step anneal may help with the tears that are forming on the membranes when they are annealed post-etch. The recipe I used was a 10 C/s ramp to 600 C, hold 60 s, 10 C/s ramp to the setpoint, hold 60 s, ramp down. I annealed membranes with…