Sensor presentation

I obtained some perplexing results today that I have re-run multiple times. Ozone treating w504 permits the passage of water in a wet/dry format w/o PVP coating. I ran several experiments with the constant pressure device with water on the top side and dry on the bottom. Some of the membranes had once been wet/wet,…

As discussed in a previous post, we decided to test chips from wafer #1070 because they have smaller pores than those used in the most recent forward vs. reverse study. In the forward direction, 31 uL of filtrate was passed after 30 minutes at 3 krpm. (after 30 additional minutes at 5 krpm the total…

This data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…

In my last post on HUVEC intercellular junctions, I just showed cadherin staining.  I repeated this protocol but also tried immunofluorescence for ZO-1, the tight junction protein. These are HUVEC P7, 6 days post-seeding @ 50,000 cells/cm2.  I looked at glass coverslips, pnc-Si and commercial PET transwells.  Since background fluorescence was so high in my…