Sensor presentation
I attached a presentation here for tomorrow’s meeting.
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Fibroblast adhesion study using controls
Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because they do not promote cell adhesion. Difference in morphology of cells could also be seen…
Lift-off Attempts for a Bone-Invasion Mimetic with Porous Roof using 100 nm NPN
This post is a continuation of the project found here: Fabricating 500 nm Channels in Silicon Wafers. The chief limitation of the previous device design was that the long narrow nanochannels were not effectively wetted. To combat this problem, we would like to fabricate the channels in situ on top of NPN. This could be…
Adsorption tests on PEG1000 etc.
I was given three groups of chips to SEM. The first group had undergone the Diazerine and PEG1000 treatment followed by incubation with either PBS, IgG, or BSA. The second ‘group’ was a chip that had been treated with diazerine and PEG1000 but had not been incubated. The last group was a chip which had…
Materials in contact with media in micropump
The pump’s data sheet (located here) describes the pumps materials as: polyphenylene sulphone (PPSU) polyimide (PI) nitrile butadiene rubber (NBR) It also says that the acceptable particle tolerance is < 50 μm. This is the info we will need to determine the types of particles we can use with an in-line flow system.
NP and IgG separations using wafer 1142 (14%, 36nm)
We used chips from the latest 5 slot SepCon NPN wafer 1142 (14%, 36nm) to test our ability to separate 20 nm “blue fluorescent” polystyrene NPs from Invitrogen from IgG. We used a concentration of 10^14 particles/ml NPs and 1 mg/ml IgG. (This is a mixture of fluorescent and unlabeled IgG) We compared forward vs…
Transwell Edge Effects – Part 2
A while ago, I posted some images of cells grown on commercial polyester (PET) transwells. Here’s the post. I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the bottom of the membrane. I contacted Corning and they said that these dead cells were…