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IEEE Spectrum: Three Artificial Kidneys…
“Three ways to build an artificial kidney” This IEEE Spectrum article list three efforts to make an artificial Kidney. The first is the AWAK, kidney on a belt. They don’t have the latest images for this, only the original very bulky design. The second is the Roy/Fissell implantable Renal Assist Device (iRAD). Both of these…
Protein separations with w415,416
I recently set up 24 hour diffusion separations with the BioRad protein standards. The following gel shows the separations using w415 position (-4,3) and w416 (-1,3). Different cutoffs were observed with the two membranes.
MgF2 grain size as a function of temperature
Summary Surface roughness is believed to impact the SERS effect. Rougher surfaces tend to produce more SERS activity. We can manipulate the npMgF2 surface by changing the deposition temperature, in the form of changing the grain nucleation, shifting the overall grain size and roughness. I performed TEM tomography and segmentation to pull out distributions of…
Nano-porous SiN Lift-off Update
Using the same successful procedure as the last lift-off attempt, another large piece of membrane lifted off. This came from the same wafer, but confirms that 30 minutes in BOE is sufficient to etch 25 nm of thermal oxide under the SU8 structure. Ideally this time can be reduced by means of a different sacrificial…
Test of Theory– Can we successfully bind Human IgG to the protein A beads and then remove it with buffer B?
Test of Binding/ Unbinding (Carrie and Barrett): Test to see if we can successfully bind Human IgG to the protein A beads and then remove it with buffer B. After ordering the new human IgG and protein A beads, I have been working on a higher-quality analysis of the binding and dissociating of the IgG…
(Lack of) Effects of Anti-CD29 antibody on endothelial barrier function
In the past, I have been working on developing mechanisms to inhibit neutrophil migration through endothelial basement membrane. Please find the previous blogs on the following links https://trace-bmps.org/updates-on-neutrophil-collagen-migration-experiments/ https://trace-bmps.org/manipulating-neutrophil-migration-through-the-vascular-barriers/ https://trace-bmps.org/sem-imaging-of-neutrophil-transmigration-through-collagen-gel/ This is a small update on the following. CD29 is beta-1 integrins, which are used by neutrophils and endothelial cells to adhere to basement…