R61: Slide Decks for 6 Month Progress Report
PDF with all slides: R61 HL154249-01(6 Month Progress)
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Simulation and PIV results of the flow module
1. Introduction Fluid flow is an influential component of vascular models for conditioning cells and introducing other components such as leukocytes. In the previous post, we described our modular design for integrating a flow module into the uSiM device. Here, the simulated fluid flow and its experimental validation using particle image velocimetry (PIV) technique are…
Monocyte Transmigration in μSiM Devices – Optimizing Microporosity for Future Studies
Introduction Infection or injury of tissue results in inflammatory signalling that motivates leukocyte recruitment to an inflamed location. Monocytes in particular are implicated in both pro inflammatory signalling through the M1 macrophage phenotype and regulation through the M2 macrophage phenotype. The μSiM devices developed in our lab have yet to assess/optimize monocyte transmigration from the…
Effect of Tau on BMEC Monocultures in μSiMs (Rotation Project – Emily Reitz)
Introduction Alzheimer’s disease (AD) is a neurodegenerative disorder that is characterized by accumulation of amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs) made of aggregates of the protein tau (1,2). It results in the destruction of neurons and their connections due to this accumulation of amyloid beta and tau (3). Tau is a microtubule associated…
Using Histogram Distance Measurements as a Means of Analyzing Flow Cytometry Data
Overview Recently, we performed a series of flow cytometry studies assessing neutrophil (PMN) activation in first generation flow devices. All the relevant data and can be found in a previous post: here. As part of the analysis, we chose to quantify relative PMN activation by comparing isotype control histograms against L-selectin (CD62L) expression histograms. This…
COMSOL simulation on sampling method to measure membrane permeability
Background We at Michigan have been aiming to perform sequential small volume sampling coupled with our ultrasensitive digital immunoassay to provide longitudinal data from the uSIM. We have encountered repeatability problems as well as low molecule abundancy with shortly incubated samples collected from the bottom microfluidic channel. Therefore, COMSOL simulation work has been done to…
Introduction of beads into the flow module
1. Introduction Fluid flow is one of the key elements of vascular models for delivering nutrients and growth factors to cells as well as introducing other components like neutrophils. In post 1, we described our modular design for incorporating a flow module into the uSiM device. Then, we presented fluid flow simulation and its experimental…