R61: Slide Decks for 6 Month Progress Report
PDF with all slides: R61 HL154249-01(6 Month Progress)
PDF with all slides: R61 HL154249-01(6 Month Progress)
Introduction Here, I will post figure drafts for the paper that introduces the modular µSiM (m-µSiM). Figures Remaining data to obtain: Occludin staining in IMR90-4 from UniBe in µSiM. We have tried this after 2 day culture in µSiM, which worked at UR but is not working at UniBe. Current problem: Occludin is not seen in…
Introduction As discussed in a previous post, we are working to adapt a PDMS flow insert as a manufacturable component. While our priority was maintaining the original flow channel geometry as Mansouri et al. designed, we slightly changed the channel length (5 mm –> 4.6 mm) to accommodate an extra bonding area for the pressure-sensitive…
R61; 12 month Progress (Rochester) Small Trans-Agency Blood-Brain Interface Program Investigator Meeting – Agenda_FINAL
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…
Introduction Pericytes are mural cells that line the outer surface of microvessels and regulate diverse aspects of vascular development and function. We differentiate brain-pericyte-like cells (BPLCs) from hiPSC clones using the protocol published by Eric Shusta’s lab (Gastfriend et al., 2021) (Figure 1). This protocol describes methods to differentiate hiPSCs to BPLCs via neural crest…
Introduction The basement membrane (BM) is a critical component of a healthy blood-brain barrier (BBB) model. Further, the BM breaks down in several diseases. Both pericytes and endothelial cells can contribute to basement membrane production. This post will explore a method to localize basement membrane components – collagen type IV (Col IV), fibronectin (FN), and…