10dynes/cm^2 Aline bottom channel

Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA microarray. The idea is to create a system to screen for drugs that break protein-protein…

Previously, we showed that our method of pll-peg coating is effective for creating non-fouling substrate which can prevent protein adsorbtion, and this method can be used to create a non-fouling pattern on the surface. In the next step, we wanted to make sure that whether our peg coating is effective for preventing cell adhesion or…

Adeel and I summarized our key takeaways from the BMES in this post. I personally tried to attend organ on a chip talks and posters, which included a micro- or nano-porous membrane or a micro/nano-structured co-culture system. I tried to identify weaknesses and strengths  to highlight our potential opportunities to contribute, and to improve our…

Art is going to make a second stir cell this week. I asked him to replicate it as closely as possible, however I’m not sure how well that’s going to work. The one we currently have is made out of Ertalyt, a material similar to teflon. Paul gave us polyethylene which is much softer than…

Today Barrett and I processed SiO2 deposition at RIT. This process was actually delayed due to the cleanroom scrubber failure last week. We deposited 30nm SiO2 on both sides of three wafers, #335, 341, and 345. Earlier in March, I tried 20nm and 40nm SiO2. It was found that 40nm SiO2 is so thick that…

Above video shows the disruption of the membrane from W404 after around 12 hours in the DMEM media (benchtop). The membrane was not treated with UV-Ozone and degraded pretty early as compared to that from W336 which broke at ~28 hrs. The membranes were loaded with 15um beads to check if they were intact and…