Developing a Microfluidic Human Tendon-on-a-Chip to Investigate the Role of the Vasculature in Tendon Injury – MPS World Summit 2024 Poster

As I was preparing for the BMES conference, I took a few pictures of endothelial cells on pnc-Si transwells that showed extensive vacuole/endosome expression (here).  The most dramatic example occurred on a 50nm-thick membrane that was barely porous.  So I wondered – are cells responding to mechanical or porosity differences (or both, or something else)?…

  These Adarza chips were pre wetted with PBS then incubated with fetal bovine serum (FBS) overnight in the refrigerator then rinsed. TRITC (2 mg/mL) added to  chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI) water. I first measured using the…

Using the techniques described in my previous post: TEM Tomography of NPN and npMgF2, we have recreated some tomograms that have contour (position) and height (depth) information contained in them. Our goal now is to express that height information in the form of a surface, or volume.         Segmentation Having some evidence that…

Silver chloride electrodes are useful for microfluidic devices since they can generate current below one volt. This allows them to function without fear of electrolysis confounding experimental results by way of gaseous clogging. Several different methods for coating silver mesh were tested to determine which would be ideal for future experimentation. These methods were as…

It was speculated that the rate of diffusion through a charged membrane had a direct relationship with pressure – as pressure increases, the kinetic energy of the charged particles is more likely to overcome the electrostatic repulsion of the membrane, until at a high enough pressure the repulsive force is small enough to be ignored….