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Forward vs Reverse Centrifugation (continued again)
As discussed in a previous post, we decided to test chips from wafer #1070 because they have smaller pores than those used in the most recent forward vs. reverse study. In the forward direction, 31 uL of filtrate was passed after 30 minutes at 3 krpm. (after 30 additional minutes at 5 krpm the total…
Discoloration Array (revisited)
I repeated Barrett’s discoloration array experiment (Array1, Array2). NaCl solution was prepared @ concentrations: 0, 0.25 (0.1), 0.5 and 1M, whereas NaHCO3 solution was prepared @ 0, 0.475, 0.95, 1.90 (and 3.80) mg/ml. Different combinations of both these salt solutions were used and discoloration of non treated samples in oven@ 37C was monitored over different…
‘HTTP error’ when trying to upload images
ByJimHello everyone, After some work on GoDaddy’s side, we have fixed the HTTP error when trying to upload images. Please let me know if you encounter this issue in the future. Cheers!
Hydraulic permeability of carbonized membranes
A simple hydraulic permeability study was performed to determine whether carbonization would negatively impact water transport through the membranes. 5 slit square SepCons from SC 471 were carbonized and put in the ultra-centrifuge with a 500 uL starting volume. The samples were spun for 10 minutes at 3000 RPM (equiv to 4.95 PSI starting). SC…
A pH vs. Zetapotential graph for SiN membranes
The blue line reflects measurements taken with my zetapotential setup. Each data point is the average of three independent measurements of zetapotential (a new 3-slot SiN wafer 1024 chip for each measurement) and the linear fit for the pressure vs. streaming potential was at least R^2 > 0.92. Error bars come from the standard deviation…
Diffusion of Apobec 3G through a pnc-Si array
I recently began working directly with Dr. Harold Smith’s A3G protein. In my previous posts, I worked with BSA and IgG in order to develop a small-scale diffusion set-up able to reach equilibrium before it dries out. These preliminary steps were working towards my ultimate design for a quick and accurate diffusion set-up for A3G….