Modeling pericyte loss in CNS disease using the µSiM platform to understand post-operative delirium superimposed on dementia – Michelle’s MPS Talk

Hey, this will be a rather short post, but kind of important for the current work on my 5637 EV capture. While working over the break, I was messing around with capturing CFSE labeled 5637 EVs and I noticed that there were many large clusters on the membrane surface (Figure 1A). This was concerning to…

The title really says it all. I have been attempting to make a better cell culture substrate with the Magnesium Fluoride Films. Previous posts have shown the overall structure of the film, and now I am trying to quantify the strength with a burst pressure measurement. In order to make a direct comparison, I needed to…

The Ozone generator works very nicely. Dave is going to be providing an in-depth user manual for us, so I’ll be somewhat brief. In this first picture, I compare an ozone treated sample against a control. Both ‘droplets’ are 3uL of DI water. The treatment protocol:– 3 min of O2 at to fill main chamber,…

Below is an optical image that shows the amount of area loss caused by the preferential EDP etch. Based on these numbers, our most recent membrane slits measure 115 um x 2014 um.

Wafer 403 was processed last week using the new thermal oxide/TEOS steps outlined in the previous posts. I took images from six positions at different radial positions on different axis. All six samples looked relatively uniform! (1,0) – 35kx (0,-2) – 35kx (3,0) – 35kx (4,0) – 35kx (0,5) – 35kx We know that the…

Type I collagen is a fibrous protein and the largest constituent of the ECM by mass. Collagen fibers are aligned across several millimeters to centimeters within load-bearing tissues, like the musculoskeletal tissue, cardiac tissue, and the cornea. Millimeter scale regions of fiber alignment are also observed in the tumor stroma and fibrotictissue. At the cell scale,…