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DLS data on A3G
These experiments were conducted during the week of 5/10/2010 – 5/14/2010. To recap on this project, Barrett and I have been working with Dr. Harold Smith in the hopes of developing a drug screening test that consists of small-scale diffusions. In these experiments, we are working with APOBEC3G (A3G), a protein-RNA complex that is active…
Update: Critical Flux Experiments, Trial and Error
Hi everyone, in this update I’m going to go over some of the highlights from my experiments over the last month. After going through several trials that ultimately ended in failure, we moved over to testing long term performance of a 2.7% porosity nano-porous membrane (avg. pore diameter of 33.2 nm) by assessing it in…
Pore Processing Continuation
In my previous post, there still appears to be a discrepency between the calculated pore statistcis and the experimental permeabilities. I explored what I could do with the pore processing to bring the experimental closer to the calculated permeabilities. I set the minimum pore cutoff higher (15nm) and tried to remove any pores that did…
Intact pnc-Si with HUVECs after 1 week
My last cell post involved pictures of HUVECs after 1 day of growth on commercial transwells and pnc-Si Sepcons. Over the past week, I’ve been growing HUVEC on commercial PC transwells and pnc-Si in the Sepcon format in order to track the TEER. To get more data out of these cells, I decided to do…
Discoloration Study with Bacterial Media
I performed a test study to see the effect of microbial media’s : LB and BHI Broth on the membrane to check for discoloration. LB Broth : Lysogeny broth,Luria broth or Luria-Bertani broth. Obtained from Sigma and made by dissolving 20g in 1 l of D.H2O and autoclaving at 121C for 15 mins. LB is…
Webb Device Prototype
Last week, I worked with Kevin to create a prototype cell culture device that could be compatible with his Raman system in Nottingham. The space constraints of the physical system are challenging: The overall working distance of the system must be less than 1.5 mm (smaller is better), must maintain only MgF2, media, and cells…
