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Diffusion tests with Nylon and Nitrocellulose
Last week I tried to diffuse Cytochrome C through our membranes onto PVDF and PES membranes to see if we can detect protein adsorption on the other membranes. The trial illustrated that cytochrome c will diffuse through our membranes and stick to the PVDF and PES membranes. I ran the experiment again, but I used…
Exosome Scale Experiments
ByAslanHello Everyone, This is another quick update over our experiments. Exosome Scale Experiments: In all of our experiments, beads are 100nm and pores are 80nm. As you probably remember, in my last post, i talked about hos the membranes are resisstive and they don’t stand these flow rates, so the flow finds another source in…
Discoloration – Cell Media
I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting from the Elder lab (rat lung cells). I did a discoloration test to see if…
Neutrophil activation on PEGylated glass surface
Hi All, This is my first blog on the NRG group. I am working on testing the biocompatibility and of PEGylated pncSi surfaces. To start with, I and Nakul began with checking the activation status of neutrophils on PEG treated glass cover slips. So here are details of the experiment we performed. Hypothesis: PEG treated…
Multi-annealing of free standing OSO stack (2)
ByJoe QiLast time I showed that the sample with multi-annealing yielded higher porosity and larger average pore diameter than the sample with direct annealing. In this post, I further investigate the multi-annealing of free standing OSO stack (20/15/10) by varying the pre-annealing time. The heat treatment process is illustrated in the following figure. Free standing OSO…
Streaming Potential Revisited
After last week’s post detailing the problems I’ve been having with my streaming potential setup, Jirachai was kind enough to come by the U of R last friday to look at my device. We banged our heads into the wall for a good two hours, but the drifting potentials and apparent insensitivity of the streaming…


