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Reducing intramolecular and interpore translocation kinetics variation by reducing initial entropy
My PhD exam was postponed (tentatively to early September but it’s up in the air still) due to an impending bureaucratic nightmare, so for the last few weeks I have been running more filter experiments, and I have discovered some interesting trends. While it is generally the case in nanopore experiments that the average dwell…
w677 Separation
Here are the results of a protein ladder separation with w677. If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that never came in contact with membrane, R = Retentate, F = Filtrate Myosin (Band at…
Thin Film Electrode Creation
I have been trying to fabricate my own Indium Tin Oxide (ITO) electrodes on coverslips for the BBB device in order to minimize the working distance for any microscopy. Currently, I am using microscope slides that are way too thick (mm). Coverslips are generally about 0.2mm thick. I am using a combination of sputter, photolithography,…
Thin (5 nm) Au layer TEM imaging on 1148 NPN/npMgF2
Intro Understanding how the Au film coats the inner part of the npMgF2 is essential to understanding how the photonic properties of the npAu/npMgF2 sensor arise. From SEM, we are able to resolve little cones of Au, but unable to see how rough/homogeneous/open the Au layer is. Our hypothesis is that these little cones of…
AFM – new tips and wafer characterization
I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out. I think they solved some of the problems I was running into over the last few weeks. Mainly, I was finally able to image entirely in repulsive mode (which was impossible with the older tips). All of…
Holey Electroosmosis
First off – OK now on to the science. Wafer 747 has a huge amount of pinholes, and in this study I wanted to see how these pinholes would effect electroosmosis. I will show results here from two experiments with these holey membranes. These membranes have a lower rate of electroosmosis than previous tested membranes. …


