Neutrophil activation on PEGylated glass surface
Hi All,
This is my first blog on the NRG group. I am working on testing the biocompatibility and of PEGylated pncSi surfaces. To start with, I and Nakul began with checking the activation status of neutrophils on PEG treated glass cover slips. So here are details of the experiment we performed.
Hypothesis: PEG treated glass surfaces prevent neutrophil activation
Test Sample: Neutrophils suspended in HBSS (no Ca, no Mg, no serum) are treated on to PEG treated cover slip
Positive control: Neutrophils, in same buffer as above, on untreated regular cover slips
Negative control: Cells suspended in serum containing buffer, again on untreated cover slips
To begin with positive control, we observed that regular cover slips activate the neutrophils as seen in their changed morphology and increased stickiness to the surface.
Most of the neutrophils seem to be spread and non-spherical.
For the negative control, cells suspended in serum containing buffer failed to show any activation in terms of spreading or losing their spherical shape.
Majority of cells seem to have retained their shape, excepting a few of them.
Finally, our sample, the cells suspended in buffer without any serum when treated on to PEGylated cover slips also remained inactivated as per our hypothesis.
Although a smaller sample size, the cells in this case seem to remain spherical and non-spread, thus showing no activation.
Next step in this regard would be to label the prominent cell surface markers which change their behavior upon cell activation and comparing those cells with the ones in our positive and negative controls.