Tendon-on-Chip Platform for Modeling Inflammation and Fibrosis in Tendon Injury

Using a wafer with a stack of Si-25nm(thermOx)-40nm(SiN)-50nm(PNCSi), lift-off was achieved on a small scale. First the SiN was etched using the standard process Simpore has developed, this also removed most/all of the PNCSi. The standard uChem process was then followed for SU8 application. A negative mask with 10um lines and 50um spaces was chosen…

In the past, we have seen allyl alcohol treatment on silicon chips helps retain a low contact angle longer than a purely plasma treated sample does. This helps us maintain gold flow in a sepcon format for a longer period of time compared to untreated or a purely plasma treated sample. Untreated samples also will…

My previous discoloration post showed that chips in the Sepcon format discolored much slower than flat chips.  A flat chip in the same well as a Sepcon chip seemed to accelerate discoloration.  One thing about the Sepcon format that I hadn’t thought of earlier was that pnc-Si in Sepcons is autoclaved for sterility.  So I…

Hi all! Above is an image of the bioreactor made from the square, 5-slit pnc-Si chip and a PDMS structure.  Fused-silica capillary tubings were inserted into the bioreactor to form the inlet and the outlet for the transfer of the cells into the lower compartment, which is made entirely out of PDMS.  Note that the…

Cell Culture on the CO2 exchange Devices  The system becomes more complicated with the addition of cells. Tejas Khire cultured HUVECS in a device that had an inverted design to the one pictured above. (The cells were plated on the top portion (well side) of the nanomembrane with media and the bottom (flat side)of the membrane was…

I recently got some good looking results on the FLAG diffusion test over a 24 hour period. The relative concentrations of enzyme seemed to indicate that measurably more enzyme diffused from retentate to filtrate in solutions not containing constricting Anti-FLAG antibody, and this is excellent news, but over the 24 hours between beginning the diffusion…