It took me a little while to figure out how to turn the .avi file into a .gif, but here is an image of the diffusion through 501. Due to a problem with MatLab, it took about 5min before I started taking images of the diffusion. So this starts at ~5 min after the 100mM rhodamine was pipetted into the well. Diffusion is much slower than what I normally see, but the rhodamine is getting through the membrane.
images at .5 min intervals, 20 images total
I’ve updated the figure from this post to add new data from oxidized and pre-washed glass samples. We now have possession of teflon material, which I will be testing this week.
w312 Number of Pores HistogramContributed Area Histogram 5nm BSA-Au is detected in the filtrate after 24 hours. 10nm BSA-Au is not. The 5nm registers around 10nm after conjugation and the 10nm is closer to 15nm. This selected membrane is not very porous, although it does have pores (as high as 42nm) that would appear to…
Introduction Over the past year, our lab has been working in close collaboration with the Schwarz lab to design, develop, and manufacture microfluidic transwells for their P50 project. The project was initiated after the discovery of S. aureus migration through sub-micron canaliculi in vivo. To further explore the genes involved in this newly proposed mechanism of immune…
Kyle has made some progress using the monolith chips, however, the wetting of these chips is difficult and makes the monoliths unattractive to use. I have attempted to create a more replicable wetting protocol. We’ve heard over the summer that other groups have used an autoclave to inundate tiny nanostructures with water (like a nano-scale…
We have seen before that silanization slows down discoloration and hence prevents membrane degradation. In Sepcon, the sample should last over a week. Further, it was necessary to check whether silanized surface supports normal cell growth. Therefore, comparison of cell adhesion on aminosilanized surface is made to control surface (non silanized). I took samples from…
A few weeks ago, Henry and I ran a migration/wound healing experiment using two cancer cell types: T24 (bladder cancer) and MDA-MB-231 (breast cancer). MDA cells are highly migratory, while T24 cells are less so. We wanted to test if the exosomes from MDA cells would cause T24 cells to become more migratory. This was…
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Where on the wafer was this membrane from?
It’s 501. I wrote it in the title but not in the main post, sorry.
Sorry, misunderstood the question. It’s position -3,3.
Don’t forget that diffusion is not a good way to judge porosity. However, it is an nice way to show if there is ANY porosity. The air permeability data indicates that this wafer is about 1000X less porous than our high porosity membranes.