Metallized Membranes

Residual matrix from different separation techniques impacts exosome biological activity (PDF) Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta & Paolo Bergese. Scientific Reports 6, Article number: 23550 (2016) doi:10.1038/srep23550 Abstract Exosomes are gaining a prominent role in research due to their intriguing biology and several…

Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the treated and untreated cases using Jess’ diffusion cell. Again 6uL was placed on the membranes…

What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure overnight) Ozone Top Electrode + Feet (1 hr, cure overnight) Weaknesses are that there are…

I used the source meter that Chris brought over and tested the voltage at points across the chamber. I set up the system with 1x PBS at 15V (~10mA) with a pinholed membrane. I tested the voltage drop at the outer edges of the well, in the center, and the inner edge of the chamber…

Summary The CEIS project ended in June, and I am summarizing the last few weeks of activities below by Josh and me.  We did a little more work on reducing the SU-8 film stress through the processing conditions.  We also succeeded in obtaining smooth poly-Si films in the RIT SMFL.  We made a few test…

On Friday(which was a great day for science). I ran a series of 5 passes of the bioconjugated gold nanoparticles through 30nm cutoff Gen 3 Sepcons. If you’ll remember, the Barcikowski lab wants the free ligand separated out from the complexes. To indirectly get the relative concentrations of gold and ligand, we measured the absorbance…