On the 13th of September we reviewed our recent progress on EO. Maryna and Jirachai (Borkholder group) gave presentations. I’ve uploaded PDF files of each of their presentations.
Maryna’s presentation:
Jarachai’s presentation:
EO_Testing_Report (PPT)
EO_Testing_Report (PDF)
In my previous post, our urea clearance for the single pass experiments was found to be ~140 ml/min/m^2, which was similar to Fissell/Roy’s value except we used a flow rate of 1uL/min whereas they used a flow rate of 10 ml/min however. To show that we could achieve even greater clearance values than their group…
Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th. Please come try out your assays on it. Or check sensitivities. Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…
We have re-started the PEG x-linking project at RIT. One of the goals is to create microwells made of PEG on top of a membrane in order to culture individual cells or small colonies of cells with the potential for a co-culture with a confluent monolayer on the opposite side. PEG is available as PEG-Diacrylate…
Over the past five days Anant and I monitored human umbilical vein endothelial cell (HUVEC) proliferation on three substrates: RTP-treated Si samples (both with and without membranes), glass (microscope coverslips), and plastic (6-well tissue culture plates). After the first two time points very few cells were observed on the silicon samples, so we added more…
After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’ red 1µm beads (in 1X PBS) onto each type of membrane (the SiO2 and SiN…
Our main purpose of this project is to reduce or eliminate the protein non-specific adsorption on the oxidized siliconnitride sample surface. By synthesis the PEG molecular on the surface, we believe we can reach the goal. There are different ways to synthesis the PEG on the surface. First is the silanes, after synthesis the silanes,…