Air Permeability Update

Roundness vs. Equivalent Diameter plots for five eccentric wafers: There really doesn’t seem to be a correlation between equivalent diameter and roundness. However, there does appear to be a rough downward trend in SC 016 and SC 258. Mean Roundness vs. Wafer Number graph with error bars included: Without the error bars, it looked like…

This is a retrial of the negative adsorption assay using the Tecan spec/fluorimeter that we have been demo-ing. To perform this assay, I am applying a known concentration to the surface I want to test. After an hour I remove some of the solution and measuring the remaining concentration using absorption and the Bradford assay….

I have had a chance to play with some of the chips from Lot 1278, specifically the design with the 2 micron slots. Results are a bit of a mixed bag. Wetting these chips continues to be a challenge. My first attempt to use the vacuum wetting protocol in ethanol resulted in a broken membrane,…

Fluorescent beads were employed to assess electroosmotic flow in various devices. The beads were 200 nm in size and were negatively charged (on account of being carboxylated polystyrene). These beads were suspended in a 0.2 mM K2HPO4 buffer. They were also incubated in 1% BSA solution in an attempt to reduce their surface charge, though…

For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording.  (reporting a low resistance: 5-10 ohms, shorted). TEER Test…

Hey all, Here’s my thesis: ksmith thesis I filmed the public portion of my defense and posted the video to youtube. I’m not leaving till the end of the week, and I’ll be back for a week in mid-May as well as being generally around Rochester this summer (this isn’t goodbye!) but my permanent email address…