Air Permeability Update

Last week, I worked with Kevin to create a prototype cell culture device that could be compatible with his Raman system in Nottingham. The space constraints of the physical system are challenging: The overall working distance of the system must be less than 1.5 mm (smaller is better), must maintain only MgF2, media, and cells…

Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3). The gel is posted below: The columns are (from left): Protein, control, retentate 1, filtrate…

In a previous post I showed that a colorimetric assay using sulfuric acid and phenol can be used to establish a linear relationship between known detergent concentrations and absorbance values. This assay proves helpful in the project related to Mark Dumont’s work in that it will allow detection of small quantities of detergents that pass…

Thanks to SiMPore’s push for TEM grids, we’ve produced a lot of amorphous material in the last month under different deposition conditions.  Below I’ve illustrated the different film structures resulting from depositing with and without substrate bias and heating. Without bias, the amorphous film exhibits clusters of thicker/denser? material (darker spots).  Intuitively, this makes sense…

This is in continuation to the studies that I published here. I used two types of membrane surfaces: nano nitride trenches (the back side of membranes) with pure nitride on the surface, and zero-second-etch nano nitride with a completely intact pncSi cap on the top. These two surfaces basically act as materials with two extreme…

  On Wednesday we ran several separations using both the EA and PEG treated membranes. The treatment process had damaged many of the chips. Of the 10 treated 10nm PEG chips, 2 survived. Of the 10 treated 15nm PEG chips, 2 survived. Of the 10 treated 10nm EA chips 8 survived. Of the 10 treated…