Today I looked at 3 more wafers:
w158 (9/7/07) – some pinholes, overwhelming amount of very fine debris, not cbs as far as I can tell
w160 (9/7/07) – some pinholes, debris, no cbs
w171 (9/26/07) – many pinholes, lots of debris, no cbs
w158 (200x) – small particlesLink to updated report.
Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells. Summary/Conclusions: In this follow up post, I seeded adipose derived stem cells on 50 nm thick microporous SiN liftoff membranes with…
I talked about this yesterday in lab meeting, but here is the data. Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that of DMEM. The control was a wafer in DMEM/F12 with 10% FBS. None of these…
Introduction The blood-brain barrier (BBB) plays an important role in maintaining brain chemistry by restricting the permeability of small molecules and cells. Inflammatory diseases may disrupt the BBB and promote the development of neurological disorders [1]. One potential mechanism of BBB disruption is the degradation of the extracellular matrix proteins that make up the vascular…
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[presentation] HUVEC migration and fibronectin
Diffusion based separation theory uses a hindrance model to explain how the pores affect the diffusion of molecules of different sizes. The simplest and most well known hindrance model is given by the Renkin Equation: where Dm is the diffusion coefficient within the membrane, D0 the free diffusion coefficient, and α the ratio of molecule…