Although most previous studies suggest a weakened cell-substrate interaction over porous membranes, there are variations in the observed cell responses, likely due to the use of different cell types, substrate materials, and experimental methods. We are seeking to develop a computational model using the Active Brownian Particle (ABP) approach to study the role of pore…
The idea of this project started when we were writing a mini-review about cell-substrate interaction. Although most of studies of cellular responses to porous membranes have pointed towards a multiphasic response with the same trend of changes with respect to the pore sizes, we also found some variation in the results. We suggested that some…
We previously explored cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on the discontinuous substrates (porous membranes and non-fouling PEG islands). Here, we were curious about mechano-sensing on these substrates. We first investigated the f-actin polymerization and focal adhesion formation of Adipose-derived stem cells (ADSCs)…
Cancer cell metastasis is responsible for 80 percent of cancer-related death. The metastatic cells migrate through tight constricting spaces, therefore constricted migration and its enabling mechanisms have received a lot of attentions in recent years. The most famous research groups that are working in this field are Lammerding group, Discher group, Wolf and Friedl group,…
We previously explored cellular behavior on porous substrates and discontinuous substrates. We have seen differences in cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on these discontinuous substrates. We were curious about mechano-sensing on these substrates. To investigate mechano-sensing on disrupted surface, we developed a…
Optimization of the PLL-g-PEG coating on SiO2 substrate: The first goal was developing an efficient method for PLL-g-PEG coating which provides a high density of PLL-g-PEG grafting on SiO2 substrate and effectively prevent cell adhesion. To achieve this goal, different concentrations of PLL-g-PEG in 10mM HEPES solution ranging from 0.1 to 0.9 mg/ml (0.1, 0.3,…
Previously, we showed that our method of pll-peg coating is effective for creating non-fouling substrate which can prevent protein adsorbtion, and this method can be used to create a non-fouling pattern on the surface. In the next step, we wanted to make sure that whether our peg coating is effective for preventing cell adhesion or…
As part of my thesis, I am trying to get a non-fouling pattern on a substrate which resembles our porous membranes to investigate cell behavior including cell adhesion and migration and to compare with our previous results which was obtained on porous membranes. This study can separate the role of pore edges and disrupted surface…