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Millipore Membrane Separation
This gel shows the separation using two 100k Microcon membranes and 1mg/mL brain extract. 500uL of 1mg/mL brain extract was loaded into the Microcon and spun at 14000g for 5min. 400uL passed through the membrane (100ul remained on top). It appears that their “cutoff” as we would consider it is much lower, more like 50kD….
EO Update
ByJimOn the 13th of September we reviewed our recent progress on EO. Maryna and Jirachai (Borkholder group) gave presentations. I’ve uploaded PDF files of each of their presentations. Maryna’s presentation: MarynaEO Jarachai’s presentation: EO_Testing_Report (PPT) EO_Testing_Report (PDF) Bubble formation in electrolytic cells
n-Dodecyl β-D-Maltoside Coating on PDMS
Today I performed a coating of n-Dodecyl β-D-Maltoside (“DDM”) on PDMS in an attempt to block protein adsorption (reference). The reference describes two different procedures, one in which the protein being incubated on the PDMS surface is in solution with DDM, and one in which the DDM coating is performed before the sample is washed…
New wafers – discoloration
Since Jess saw discoloration last week within a day in PBS with the new material, I did a couple of discoloration studies this week. I looked at wafers 670 and 673 and compared them to 625, 704 and 680-T. These were not RTP’ed, I did the tests at RT since that’s the temp diffusion studies…
HUVEC Cadherin Immunofluorescence (1)
One of the markers of endothelial monolayer maturation/barrier function is the expression of adherens junction and tight junction proteins. The adherens junction is composed of transmembrane proteins called cadherins (among other proteins) that link cadherins of neighboring cells to the actin cytoskeleton. They are found in most (all?) vertebrates and are necessary for cell-cell adhesion…