MPS 2024 slides-Investigating Impact of a “Cytokine Storm” on Astrogliosis in Human Blood-Brain Barrier in a Microfluidic Model of Sepsis

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I recently started up another BBB co-cultures and I had a couple of leftover samples.  I decided to use one for a ‘just-to-be-sure’ experiment since I realized that I never did a calcein-Hoechst 33342 co-stain.  That is, I never used a nucleus-specific dye with calcein to confirm that the black holes (vacuoles) in the green…

I used the spectroscopic ellipsometer to measure several wafers deposited using the AJA system.  I verified film uniformity as well as film quality using index of refraction as the metric.  The term “w/ Bias” refers to an applied RF power over the substrate.  The bias adds energy to the surface as adatoms re-arrange during deposition…

We have previously tested the effects on filtrate volume after a given time as a function of concentration. Moving forward we want to quantify the improvement in filtrate volume when using reverse centrifugation instead of forward centrifugation. Initially, we attempted to create similar plots to those in the preceding post but got the following gobbledeegook:…

I constructed a plot of hydraulic permeability based on the flow rates that Sterlitech has predicted for their membranes. Looking at the permeability values that I posted last week, outside the tested that appeared to leak, there was a 0.01um membrane with a permeability of 33.4, two 0.05um membranes with permeabilities of 59.9 and 71.1,…