ICNMM Presentation

In our prior work we showed that endothelial cells form far fewer focal adhesions on microporous SiO2 membranes compared to non-porous SiO2 and tissue culture plastic (see prior post by Ana). We performed this analysis at 24 hours, which seems to be the standard in the literature. Interestingly, we found that the number of FAs…

Art is going to make a second stir cell this week. I asked him to replicate it as closely as possible, however I’m not sure how well that’s going to work. The one we currently have is made out of Ertalyt, a material similar to teflon. Paul gave us polyethylene which is much softer than…

I’ve made some modifications to my V2 design such that it allows total vertical access, among other things:   This design utilizes embedded electrodes and filter within gasket layers to create a tight fluidic seal for each channel. Here, the slot is pointed upward, so the endothelials will be on the bottom side and the…

I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them.  From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…

Overview: Bob etched 4 non-porous membranes, on the backside of the membrane. HUVECs were to be grown on these etched non-porous SiO2 membranes and compared to unetched non-porous membranes. Below Bob describes how the membranes were etched: “Four of the non-porous SiO2 membranes were placed on a ø6” carrier wafer with the membrane facing up in the…