ICNMM Presentation

The question arose last week about the affects of transport with use of the shaker in the Tecan, which led to a discussion about the current construction of the sepcon. The sepcon layout currently is as follows: The o-ring is 1 mm in diameter. Is this difference between the membrane and the receiver fluid dead…

This is a follow-up post to “Protein Assay Test.” I realized that I never posted the final stag of my experiments, in which I finally got BSA to go through the membranes. In “Protein Assay Test,” I described several experiments that I set up to see if I could get BSA to pass through a…

Summary: The fabrication of NPN relies on the formation of pnc-Si over a non-porous SiN film, then etching the pattern of the pnc-Si into the SiN. I used the same concept to replicate the dual-scale nanomembrane style (microporous+nanoporous) featured in Alec’s work, as well as create a porous/nonporous nanomembrane substrate, by using existing SiN or…

We now have a linear standard curve for sodium fluorescein (Na-F) in clear 24-well plates.  What is the detection limit? The detection limit is defined as the lowest background-corrected concentration that is 3 standard deviations above the noise of the background.  The background here is HBSS with 10mM HEPES. I measured the dilutions a four…

It was decided during my rotation-review meeting to use a smaller PEG molecule (3-mer) for silanization of pncSi. Following is the brief description of the polymer.   Boiling Point: 147-9°C @ 0.3mm               Freezing Point: <0°C Specific Gravity: 1.16                                    Vapor Pressure, 25°: <0.1mm Vapor Density (air=1): >1…

  The NPN tenting mechanism I’ve described in a few different posts has relied solely on mechanical technique. I poke the substrate with tweezers, or delaminate it with high electrical forces, or heat it up and let it contract while in contact with each other.  It would scale very poorly if we tried to make this…