stir cell

Invitrogen offers a number of stains and dyes for use in tracking cells and fluorescent imaging of tissue culture.  One prevalent live stain is called CMFDA (5-Chloromethylfluorescein Diacetate), a green fluorescent molecular probe.  This stain is utilized for tracking cells and monitoring growth & viability of the cells. While performing studies using CMFDA, there appears…

Previously I have silver stained all gels because the separations look very clear with this staining technique.  However, the silver stain is not giving very quantifiable results.  Coomassie staining means a loss in sensitivity, but it’s the gold standard for quantifying protein amounts.  Here I’ve stained one of the protein separation results and rerun with…

Now we already confirmed that pores are able to form in both oxide/silicon/oxide and nitride/silicon/nitride system. Here comes an interesting question- how about nitride/silicon/oxide and oxide/silicon/nitride system? In the past week, another two different stacks including SiN(30nm)/ a-Si(25nm)/SiO2(30nm) and SiO2(30nm)/a-Si(25nm)/SiN(30nm) were tested. The nitride film was deposited with 25 W bias and the silicon with…

As Aim 1 of my thesis involves using theoretical models to be able to predict particle capture, it is important for me to fully understand how each model system behaves when compared to experimental results. One of the most important of these systems is the pressure drop across the membrane in our devices. I have…

We desire to test the electrical perfomance of our membranes in the iBidi chamber. For comparison, I am using a gold standard Ussing chamber format to examine the contribution of our membranes to the overall frequency response of a system. I am using both a matlab model and a web-simulator (www.circuitlab.com) to examine an equivalent circuit model…

One of the major goals of my work with capturing exosomes is to show that our purified population is less contaminated with protein than exosomes purified by ultracentrifugation. This is important because the protein (and all the other junk floating in plasma such as cfDNA) can cause problems with identification and characterization of the exosomes….