In a first attempt to reduce the electrostatic drag that might be slowing water transport, I ran an experiment with 1x PBS. The membranes were first ozone treated and then assembled into the SepCon inserts.
From these first trials, it appears that PBS does pass through more quickly than DI water.
In collaboration with Alex Shestopalov’s lab, I started working on liquid based PEG treatment on windowed sepcon chips. The treatment is a 3-day long protocol and involves multiple washing and drying steps with different reagents. Most of our sepcon chips broke down in first few steps itself. The initial wash consists of Nanostrip (sulfuric plus nitric…
In prep for manuscript writing I came across a paper that says they’ve created a super efficient EO pump that uses extremely low voltages. Their top flow rate (.125 mL/(min V cm2) ) is comparable to the flow rates I see with our materials (144 mL/(min V cm2)). They run their experiments at 3 V…
In an effort to imitate Sepcon, I applied nail polish on two samples covering the edges and the back side (well side) and compared its discoloration to Sepcon format and w/o polish sample in 2D format. I used two polished samples in which I covered the edges in both but the back side was not…
When we submitted the EO paper way back one of the reviewers made the following comment … What is the “head pressure” P_max, i.e. the maximum back pressure that would stop the pump? For any linear electrokinetic phenomenon such as EO pumping, the flow rate decreases linearly with backpressure until a certain point (“head pressure”)…
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Our first attempt to “reverse” centrifuge materials using our standard 5-slot filters made of NPN from wafer 1070 are detailed in a previous post using GEN2 SEPCON assemblies. In this post we modify our method to more closely match the Sartorius Centrisart approach. To that end, we switch to using GEN3 SEPCONs because they are easier…