Congrats Dave!

Our previous work with reverse centrifugation NPN membranes has shown that they may work for cleaning up excess protein from conjugated NPs.  This initial success used ~20 nm red fluospheres with membranes from wafer 1070 (10-15%, ~45nm) and unlabeled IgG we were given by a collaborator.  In this post we are testing “newer” NPN wafers…

Some background and a quick summation since I have done a great job at avoiding posting. My undergrad background before the lab Chemistry, physics, math (minor) and a random psychology minor biology I questionably knew what a cell was Overarching theme Putting a microarray on top of the membranes for cell culturing Currently cells are…

Links and Papers: Article with video Lung on a Chip Device Paper Lung on a Chip Application (Pulmonary Edema)   Chris came across the publicity video last week and Jim asked me to look into the specifics. It’s a pretty nifty device. As the video shows,  the device is a co-culture structure fabricated entirely from PDMS….

Objective: Determine the minimum required area and number of exploratory cytoplasmic protrusions for sensing ECM alignment and causing cell polarization. Hypothesis: Cells only need to sense collagen alignment through exploratory protrusions extended during initial phases of cell seeding and not throughout the entirety of the cell, and a minimum number or size of protrusions is…

To prepare the BBB devices for cell culture, they must be sterilized. To that end, I wanted to see if there were any adverse effects from autoclaving the device. Recipe 4 on the U of R autoclave is a dry process that we use to sterilize tips, so I used the same recipe. I placed…

I am working with different electrode materials as a substitute for ITO for my TEER measurement device. I used silver-silver chloride electrode and platinum-iridium ribbon (90:10) for that purpose. I made AgCl electrode by dipping about an inch of silver wire in silver chloride ink and letting it dry for half hour. I obtained the…