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Adsorption Studies
The following changes were made in this adsorption study: The MicroCons/NanoSeps were not spun. We will just characterize the differences between the materials. The pnc-Si membranes were assembled into SepCons because of the potential adsorption to the plastic surfaces. Mass standards were run on the gel in order to allow the measurement of protein concentration….
Merging Man with Machine: A Computer Vision Aided Leukocyte Transmigration Assay
Introduction High throughput imaging techniques in conjunction with in vitro micro-physiological systems (MPS) allow for comprehensive exploration of physiologically relevant phenomena in a living 3D mimetic of a tissue microenvironment. Prior work in this lab has readily demonstrated that vascular tissue mimetics (the µSiM) facilitate real time phase-microscopy visualization of immune cell trafficking in response…
A closer look at separations
UPDATED 12/12/07 – Added data on albumin. In these gels, the band from beta-galactosidase appears to overlap with phosphorylase b making it difficult to determine which protein passes and which is retained. I have used Mike Bindschadler’s pore imaging software to find the pore sizes of the three membranes I have use to perform succesful…
Organic-Inorganic Hybrid Membranes Formed from Block Copolymers and Titanium Dioxide Nanoparticles (A Review)
The Wiesner lab at Cornell (whom you may remember as the discoverer of the neutrally charged but highly fluorescent “C-dots” Jess used for her charged-based separation work) are about to publish Asymmetric Organic-Inorganic Hybrid Membrane Formation via Block Copolymer-Nanoparticle Co-Assembly in Nano letters. Building upon their previous work on block copolymer self-assembling films, they have added titanium dioxide nanoparticles…
Etch cell troubleshooting
As many of you know, we have been having a lot of trouble with the new etch cell and have not been able to produce high quality membranes. Yesterday, we discovered that the stainless steel used to fabricate the new cell is different than that of the original cell. Here are the EDAX spectra: Old…
Adsorption Quantification III
UPDATE: Teflon data now include Original Post: Refer back to Part I, and Part II This weekend I ran a bunch of gels to flesh out the adsorption data a bit more. I tested MicroCons, NanoSeps, and teflon and reran the initial Pnc-Si gel to get rid of negative results due to uneven staining. To…
