Author: Sean Bellefeuille

What is SiR-DNA? It is a far-red DNA stain, known as SiR-Hoechst, and can be used for live-cell imaging. Most current DNA dyes are toxic or affect the cell’s motion or proliferation. https://www.nature.com/articles/ncomms9497 It does not affect the migration of human dermal fibroblast cells (hdFb). The motility parameters (speed and displacement) for a 24 hour…

For the past few weeks, Henry and I have been working with human dermal fibroblast cells and 3T3 mice fibroblast cells. We have been working to quantify wound recovery in hopes to be able to incorporate exosomes from different cell lines to see the effects on wound healing. The NIH 3T3 cells are small cells…