Introduction/Methods As part of our investigation into neutrophil transmigration on tissue chip systems featuring HUVEC monolayers, I have been working on developing code for both cell state analysis and tracking using Wolfram Mathematica (and occasionally Python). This project relies on semantic segmentation via machine learning and microscopy video corrections, both of which have been described…
Introduction Phase-contrast microscopy is widely utilised in live cell imaging, with pixel segmentation protocols in particular finding widespread usage as a means to computationally isolate cells from a tissue-culture background. Computer segmentation allows for the analysis of individual cell properties such as state or spatial location through automated or semi-automated techniques, ultimately attempting to avoid…
Introduction Selective leukocyte interactions with endothelial barriers are a characteristic component of the inflammatory response process. These spatio-temporal interactions can mechanically remodel local tissue, as well as damage healthy tissue with robust inflammation stemming from an over-reactive immune response. This is evident in many pathologies and can complicate severe conditions such as sepsis. Thus, it…