µSiM permeability

memBrains | NRG

Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach)
ByJim January 27, 2021February 23, 2021

NOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on application in the R61 project. Small molecule diffusion from the well to the channel side…

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memBrains

10 kDa Small Molecule Permeability Optimization
ByMolly McCloskey November 15, 2020February 9, 2021

Introduction A healthy blood-brain barrier is not permissive to most small molecules, but during inflammation, tight junctions weaken and the barrier becomes more permissive. We have developed an in situ permeability assay that tracks diffusion across a cell monolayer and membrane and into the bottom channel of the µSiM device. The assay tracks a fluorescent…

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