I found some good descriptions of hemodialysis experiments using rats. They all use a method possibly first described by (Rockel et al., 1978). (Galons, Trouard, Gmitro, & Lien, 1996) at U of Arizona used nephrectomized male Spraque-Dawley rats (Holtzmann Co., Madison, WI) weighing ~300 g to study the effects of hemodialysis on the apparent diffusion…
I ran a 30 hour urea clearance with 100% serum through the single slot dialysis device. The now standard protocol was run. The single slot chip was annealed in the RTP for robustness then clamped into the acrylic/PDMS flow fixture. Ethanol was pumped through the chip for 1 hour, then degassed PBS was pumped for…
I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are shown below. W520 is weaker than 558 and 559; I plan on using the stronger…
I recently received wafer 567 from Dave for cell culture work. It has many intact membranes but is riddled with pinholes, having only 3 pinhole-free samples. I decided to test them for air permeability and burst pressure, shown below. I also noticed some odd features on the membrane and surrounding Si. They are circular but…