I recently received wafer 567 from Dave for cell culture work. It has many intact membranes but is riddled with pinholes, having only 3 pinhole-free samples. I decided to test them for air permeability and burst pressure, shown below.
I also noticed some odd features on the membrane and surrounding Si. They are circular but do not seem to be completely through the membrane like pinholes. I circled the feature in the images below.
Thanks to our collaborators at the University of Chicago we have now used NPN to demonstrate sodium/hydrogen exchange functions at the level of single EVs. This is first-of-a-kind evidence of protein function in individual EVs. It will be officially published in Communcations Biology and will soon be located at this DOI. What follows are key…
We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or cell spreading/contracting. Josh Miller made new Microporous and Nanoporous Nitride liftoff membranes. These membranes were…
Hello Everyone, I have been working on 100 and 50 nm beads since ACNMM conference to optimize our isolation technique for exosomes and verify that our approach works. Technically, based on the discussion that we had before about Peclet Number, in order to decrease this number, I have been running all my experiments with 1…
In order to figure out the relationship between grain size and pore size, I tried to use XRD measurement to determine the grain size by Scherrer equation. t = kλ/(B cosθ) where t is the average grain size, k is the shape factor that is in the range 0.87-1.0 (it is usually assumed to be…
A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid. Image was taken right after sample preparation. Xiao
In order to resolve the silicon nitride- silicone gasket bonding issue, I, Josh, Tucker and Greg used different approaches to functionalize the nanoporous nitride (NPN) surface. Both surfaces of NPN, although have same material (silicon nitride), behave differently. The flat side is etched using xenon fluoride to remove pncSi layer on top. Different tools at…
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Karl,
The feature you circled looks like a contaminant, probably from the Pentane dip.
Thanks Dave. I forgot to mention this is a single slit wafer with 100um x 1000um slits.