Journal club

So in the past couple of months, I have focused on wrapping up the microdialysis project that was initiated by Alec. Just as a recap, this experiment utilizes a modified SepCon that features an open bottom to allow for separation of albumin and cytochrome c via diffusion. 50 uL of 1 mg/mL BSA and cytochrome…

This week I’ve added male connectors to my device. Originally, I had female-female tubing connections to the device, which were very prone to leaking around the insertion ports. Tejas suggested that I try printing out the male connectors to combat this problem. The tubing I’m using is ID=2 mm, OD= 5mm. It’s compliant enough to…

A paper by Munch (from Kodak), Zestar, and Anderson in J Mem Sci (1979) writes that charge has a big effect on albumin separations in track etched membranes.  They show in their paper that they can make the theoretical sieving match the results better if they simply add the Debye length to the molecular radius…

In the wake of the apparent loss of all of the phosphatase in a sample that was placed into a CytoVu well, left for 24 hours, and then checked for phosphatase activity, it’s become clear that the adsorptive qualities of the CytoVu assemblies needs further characterization. I showed in an earlier post that there was some loss…

I used 100kD Millipore Celllulose membranes to separate the 2 C-dot particles.  The results are similar to those I’ve obtained with pnc-Si; the 10nm particles pass while the 30nm ones do not. Cy5 10nm particles: Sulforhodamine 30nm particles: Here are the sieving ratios: Particle Ratio 10nm (abs) .60 10nm (fluor) .59 30nm (abs) .02 30nm…