Previously I had posted a chart depicting the permeabilities of SepCon membranes from w310. I went back and noticed some errors in the equations. Here is an updated version of that chart. Also included is a sample where I used the ultracentrifuge to clarify the protein right before the experiment, which should remove any larger aggregates. 
After integrating my thin film electrodes into the layer stack of the BBB device, I want to make some TEER measurements. I stripped off the well-plate attachment off of the TEER tool, exposing the individual leads. To test that the circuit functioned properly, I placed the leads in DI water and then…
To improve our knowledge of pinhole formation I have started counting pinholes on W510, W512, and W513. I also am playing close attention to areas surrounding the samples that have been completely etched, to see if this correlates to pinhole number. A schematic of W510 with number of pinholes is shown below. I would like…
Introduction In this post I will review work I have completed to further characterize the CAD-LB platform. In this set of experiments combinations of nanoparticle species were used to define colocalization limitations. More specifically, two nanoparticle species each labeled with a unique fluorescent dye (red or blue) were used to simulate pan EV and antibody…
Last week, I deposited silicon nitride/ amorphous silicon/ silicon nitride stack and then annealed the free standing stack after etching the substrate. The nitride film was deposited with 25W bias and the silicon film with 5W bias. The thickness for the nitride film is 30nm where it is 25nm for silicon. One of the initial…
Introduction As what we’ve learned from the previous “Catch-and-Display Immunoassay” (CAD-IA) trials, we switched to an antibody pair from OriGene with low non-specific binding ability to each other and start to conduct ultracentrifugation for all reactive reagents for the reduction of protein aggregations. However, we found an inconsistency of co-localization percentage of immunocomplexes and beads…
This previous post contrasted how gold could be separated by pressure in deionized water, but not by diffusion (w199). I checked up on that diffusion sample again after 6 days, and while the retentate had mostly evaporated, there was no gold within the remaining filtrate. As you may recall, there has been some discrepency with…