New sputtering system
The new sputtering system is scheduled for delivery at the end of June. It will bring several advantages over our current system; which translates (hopefully) into stronger and more consistent material. 75 days…
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Summary of diffusion through a pnc-Si array in a 384 well plate
During the past few weeks, I have been experimenting with different array setups for diffusion. I have been working with BSA, but I will move to APOBEC3G protein once the arrays Barrett designed are finished. All of the pnc-Si arrays used were treated with UV-ozone. Treating with UV-ozone cleans the surface and helps prevents the…
UV/Ozone cleaner operation reminder
ByJimMaryna told me the UV/ozone cleaner did not work (the blue light in front of the chamber did not light up) when she was working with Dave today. I quickly checked the machine and it is actually working properly. The trick is to make sure you trigger the “safety lock” behind the chamber before you…
Devices for Geldbard Group Initial Ideas
Sealing Patch Clamp Chips Canadian Patch Clamp Chip I have been thinking about Handy Geldbard’s group and what would enable them to study groups of synapses. Currently, I am in discussion with Jennetta Hammond and Pat Lu trying to understand some of their needs and wants. First, I considered their use of small microchannels…
Pressure Cell Update
This past week I ran multiple experiments using the pressure cell and sep-cons. I used membranes from W684 for the sep-cons since the burst pressure of these memebranes is high (around 3.65-10Psi). I added 100uL BSA to the Sep-Con and put 20uL 1xPBS on the backside. Before adding the NC to the sep-con, I assembled…
Permeance and Hydraulic Permeability of ~500 nm pores in 5-slot chips
Permeance Testing Sepcons were built with 500 nm 5-slot chips. Permeance tests were performed on Samples A-C. Nitrogen gas was pushed through the membrane at a given pressure, and the height on the flow meter tube (green graduated column pictured below) was recorded according to the location of the center of the silver ball. No units…
Consistency with Effective Field
In prep for manuscript writing I came across a paper that says they’ve created a super efficient EO pump that uses extremely low voltages. Their top flow rate (.125 mL/(min V cm2) ) is comparable to the flow rates I see with our materials (144 mL/(min V cm2)). They run their experiments at 3 V…