HUVEC on pncSi

This is the protocol I use to measure band intensities. At the end of the explanation I will post and review my recent results concerning silver stain densitometry. First load a digitized gel into ImageJ Select Image>Type>8 bit to make it black and white. Select Invert to make the bands have positive intensities. Go to…

Continuing the project that Mark had been working on, I have made some progress towards electroosmosis. Specifically, I was able to observe electrolysis through an intact membrane. Hopefully I will be able to observe electroosmosis through the movement of microspheres or nanoparticles through the capillary tubes. I tried a few variations of Mark’s design using…

Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has been a lot more labor intensive. Using a neural network classifier (Weka, out of a…

In all of my experiments involving vacuoles, I’ve seeded at 50000 cells/cm2 which gets the cells to confluence after ~ 1 day of growth.  I’ve always seen vacuoles with these parameters.  For this experiment, I wanted to see what the cells would look like after 1 day but at a much lower seeding density.  The…

Jirachi has been working to make thin nafion films using our membranes as a support structure. Nafion is polymer that contains a sulfonate group, and as a result acts as a proton wire, allowing for cations (+) to be transported across a membrane, but not anions or electrons. Such a membrane can be used for filtering a…

A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given that there is a parallel project with membrane masking of ECM with parylene stencils, we…