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Pore Processing Software Updated in MATLAB App Designer Language (v0.7)
MATLAB updated the way that it creates GUI applications back in 2015, and it is increasingly becoming more functional. Over the years I have been here, we have had more than a few requests to use our pore processing software. I have sent it out, but it’s not as intuitive for some people to use…
Hellmanex III creates liftoff membrane?!?!?
While I have been in Montreal with Albert Kamanzi and Sabrina Leslie, Albert mentioned to me a cleaning protocol that appears to strip off NPN. 5 mL of Hellmanex III in 100 mL of DI water Placed in heated water bath, covered with parafilm (setting 50C, actual 40C) for 2 hrs Delaminates NPN? The chips are mirror-like afterwards, indicating…
New Alt. Membrane Release Recipe (Nitride RIE)
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch runs usually have uneven power or power spikes during the run. Increasing window size (active area)…
YAP/TAZ Passage One Staining
After reading and discussing a paper in journal club that talked about cells having a “mechanical memory” (found here “Mechanical memory and dosing influence stem cell fate“), we decided to replicate our previous YAP/TAZ experiments with passage one HUVECs direct from Life Technologies as to avoid making our cells “remember” that they had ever been plated…
SU-8 Shrinkage
Goal: Measure SU-8 shrinkage that have gone through various soft and hard bake conditions To measure the shrinkage, the SU-8 scaffolds needed to be lifted off, but there was some issues. Originally, the SU-8 was spincoated onto an oxide layer, which would be etched away using BOE. The SU-8 had appeared to be disintegrating due…
PDMS-filled wells don't increase sensitivity for HMM detection
This post is related to the HIV drug screening project that Carrie has been working on. To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes from smaller protein monomers/multimers. Small molecules that break down the HMM form of this protein…


