Wafer 416-T

After a lot of trial and error with the hinge, I was able to create something usable!!! Apparently I cannot upload the .stl file or .skp file to the blog directly for “Security Reasons”   I printed this one out of Makerbot ABS at 228 C (build plate 115 C), with 0.15 mm layer height slicing,…

I have been working with Barrett to test two different assays with BSA. One is a standard procedure for microtiter plates and the other is a microassay procedure for microtiter plates. Here is a brief summary of each procedure: Standard Procedure for Microtiter Plates– linear range is 0.05 mg/ml to 0.5 mg/ml Prepare a diluted…

In working on a long over due paper by Barrett on endothelial response to high permeability material … Key figs … Figure 2 Figure 4 Figure 5 And one post from Nakul. I came across this highly relevant paper Pedrigi(Overby)EER11

BBB On Chip (Griep et al 2012) Jascha sent me an article about a BBB device. Their device follows a very similar structure to that of the Booth and Kim paper, but closer to the optimized design that I created, using very thin, wide channels to generate a lot of shear stress (100 micron high,…

First off, a quick update on the state of the lab. The day finally came, we had to pack up and put everything in storage, so unless there is a very pressing need, I’m done data collection for the time being. I don’t think that will be a problem; we have enough for publication as-is,…