Nanopores May Eliminate Current Evaluation Processes Used for Detecting Epigenetics-Related Diseases

I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells.  Here’s a post of images.  I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …

The Burns-Zidney paper uses streaming potential to measure the zeta potential of polymeric membranes.  This means that polymeric membranes should also be capable of performing electro-osmosis, essentially the opposite of streaming potential.  For more on this, I have a knowledge page that I’m working on entitled Electrophoretic Cell. I punched out two 30kD cellulose membranes…

In my last post, I concluded that switching the o-ring position from below the membrane to the top in the sepcon produced no leaks. The reason for this switch was to reduce the 1mm “dead-space” (created by the o-ring) that adds to the unstirred water layer (UWL). The next thing that Barrett and I looked…

The following are TECAN spectrum of the filtrates (and a spectrum of unconjugated peptide) for a series of sequential separations performed on the Barcikowski peptide (CE12W) using 10, 20, and 30nm cutoff Sepcons performed last weekend. Note that all of this data was taken with the quartz cuvette not inserted fully into the TECAN as…