Kim(Haynes)AnalChem12.pdfSimilar Posts
Cell Culture on Gradient Microporous-NPN™
ByAlecIntroduction As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress. Methods HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected…
Porosity vs Discoloration I
We have known, W683: no visible pores; W684: very few pores; W685: usual porosity. After Vlassak’s view on discoloration, I took samples from above wafers to check if any difference in discoloration can be seen as they have different porosities. We could expect the more porous material to degrade faster than the less porous and…
Making nanopores in NPN tent assemblies
My first attempt at a nanopore fabrication/DNA experiment in NPN assemblies was interesting but ultimately unsuccessful. As I said in our last meeting, I have been able to reproduce the membrane transfer process (using some steam between the two membranes to make good contact) and the wetting behavior using a freezer to condense vapour on…
Changes in nanocrystalline structure due to mechanical stress
One of the things that I’ve been working on recently is trying to explain the extreme elasticity of the pnc-Si material which is connected to its nanocrystalline structure. There are two parts in this question. First, how are amorphous or crystalline phases involved during stretching? Second, does the crystalline phase change under application of mechanical…
W502 and 504 Testing
W502 and 504 were tested for burst pressure and air permeability. Only one sample from W502 was tested for burst pressure (the other broke while setting up and I wanted to conserve samples) with a burst pressure of 4.46 psi. The burst pressures for W504 ranged from 2.06 to 6.57 (n=3). Air permeability is shown…
Microfluidic Dose Response Assay
Sugiura 2010 (Anal. Chem) This paper details a PDMS microfluidic system to perform dose response assays (what I’m hoping to achieve over this summer). They use serial dilutions to get separate trials spanning six orders of magnitude, showing an optimal dosage to kill HeLa cells. Their system is very efficient, using very shallow channels and balancing the…