A Video Showing that Breathing on the Backside of a Pressurized Sepcon Begins Flow

Tom came up with this idea of using these membrane with precise thickness and pore size control to obtain a critical thickness and pore size, beyond which neutrophils cannot go transmigrate. N-Formylmethionyl-leucyl-phenylalanine (fMLP) was used in the lower channel to serve as a chemotactic factor for transmigration. Additionally, for membranes with pore sizes or thicknesses…

In this edition of the particle capture saga (this will eventually be an epic like Gilgamesh or Beowulf, we will start telling this tale by the fire and eventually thousands of years from now scholars will look back on this work and deem it a huge impact in the history of human evolution), I have…

My PhD exam was postponed (tentatively to early September but it’s up in the air still) due to an impending bureaucratic nightmare, so for the last few weeks I have been running more filter experiments, and I have discovered some interesting trends. While it is generally the case in nanopore experiments that the average dwell…

We’ve got pores! Unfortunately, we are still being plagued by an unknown contamination that seems to be “eating” away at our membranes. This would explain why we’ve been having a hard time separating. We also observe a strong radial dependence in porosity AND size with these RT deposited films. In the past it was limited…

One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was an effect of overseeding or if cells were migrating to the freestanding area. To this…