As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress.
HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected following 5 hours.
Summary My analysis of npMgF2 has relied heavily on both tomogram and segmentation processes. The tomogram generation has thus far been sufficient, giving enough detail to our eye to identify different structures, but turning the tomograms into 3d models has been a lot more labor intensive. Using a neural network classifier (Weka, out of a…
As discussed in a previous post of mine, I am currently working on a project to develop an in vitro mimetic of the osteocyte lacuna-canicular network (OLCN) to improve the ability to predict Staphylococcus Aureus invasion and colonization of the OLCN during osteomyelitis. The purpose of this post is to provide more background behind Aim…
With some of the recent high porosity membranes being produced, I wanted to demonstrate that we can use ALD to create a movable cut-off without sacrificing too much in permeability. I took sc713 and coated it with 5 nm and 10 nm of Al2O3 i.e. I shrank the pore diameter by 5 and 10 nm….
This is a followup to my previous post, which can be found here. Previously I reported that fluorescein diphosphate (FDP) incubated on the surface of an NPN chip would adhere well enough to be visible on the membrane after gentle washing and treatment with a fluorescence-activating enzyme (alkaline phosphatase, AP.) I also reported that pre-incubation…
In the last production cycle, there were several wafers annealed for longer than our standard 1 minute RTP soak time. Recall in my earlier post, I observed a significant change in pore morphology in a 30 nm thick silicon membrane annealed at 1050 C for 1 min/5 min. In this series of experiments, we observe…
Coming to a basement near you by the end of 2008 (assuming no delays that is). Pretty exciting in my opinion.