TEM Tomography for the Identification of Nanopores in Silicon Nanomembranes
Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far:
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Are those pinholes or micron-size pores?
ByJoe QiHello everyone! After a long time waiting and setting up, the new AJA and RTP at Vanderbilt University now are ready to go! Here are a couple of pictures of the new AJA and RTP. I have been working on testing the new AJA for a while with my old recipes. The new AJA system…
Modular µSiM Device Assembly
Introduction We are aiming to make device assembly easy and quick, particularly for distribution to non-engineering laboratories. We are collaborating with ALine to develop assembly via pressure-sensitive adhesion techniques. Assembly Process Modular Device Assembly Animation2 We made ten devices with porous membranes and ten with non-porous membranes to show no leakage. Current Issues and Potential…
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More details on INT cartridge's "desalting" column
First things first, the desalting column is not intended to desalt. Apparently that’s just an old name that has carried over. The current purpose is to separate viruses from large debris after sonication. The sample in a buffer solution will be placed in the center of the cartridge and sonicated in the “glass bead pod”….
Carbonization and Discoloration, final time point
A few weeks ago, I posted discoloration results on carbonized membranes here. It was 6 weeks yesterday since I started this experiment, so I decided to stop it (few, if any, cell applications require 6-weeks of culture). These were SC141 chips in the flat (not transwell) format. I also used DMEM with high NaHCO3 levels. …