TEM Tomography for the Identification of Nanopores in Silicon Nanomembranes
Kilean and I are working on submitting a paper to Membranes on some of our electron tomography work. Here’s some of the work that we’ve done so far:
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Effects of Allyl Alcohol on Gold flow in Centrifuge
In the past, we have seen allyl alcohol treatment on silicon chips helps retain a low contact angle longer than a purely plasma treated sample does. This helps us maintain gold flow in a sepcon format for a longer period of time compared to untreated or a purely plasma treated sample. Untreated samples also will…
HUVEC Adhesion with Controls
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or possibly some other unknown factor. Adhesion tests are continuing, with the use of solid Si…
Neither Centrifugation, Sonication, nor Filtration Fix the Aggregation in the Barcikowski Lab Samples
In early May, the Barcikowski lab shipped us four samples of gold nanoparticles for us to run separations on. They took forever to get here, and had aggregated and precipitated by the time they arrived. About a week ago, the lab sent another batch of four samples (which differed slightly from the samples they sent…
First Thoughts on ECMO
In preparation for the ICNMM conference next summer (and more immediately, the paper they’ve requested by January) I’ve started thinking about what our membranes can offer extracorporeal membrane oxygenation, or ECMO. This post will begin with some background information that I think is particularly important and progress into what work I’ve done so far on…
Success with PDMS-Glass Bonding
ByMike HYesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room temperature). Once the PDMS was cured Henry used the UV Ozone system to again try…
Tom's Latest Gold Separation Figure
ByJimI asked Tom to add gold PES separations of NPs to his earlier figure and to evaluate the loss of NPs during the use of these filters compared to pnc-Si. Here is what he came up … All experiments (including pnc-Si) were done at 10 PSI with 200 ul of starting material above the membrane…