Temp-controlled 'nanopores' may allow detailed blood analysis

[NOTES] BMES 2017 highhlights

Before starting my transport project with the use of the Tecan, it was important to see how the Tecan worked with the sepcon and transwell kept in the well plate. The goals from this experiment were to: 1. Test for fluorescence from the plastic. 2. See if fluorescence from the apical side has an affect…

The group in the attached paper have found a way to form pnc-Si using PECVD.   They use a 300-15-300 nm OSO stack with a 850 C (50 C/s, 30 s) RTP to form ~13 nm pores.  SiMPore attempted this in the past, but was not able to form pores.  Using PECVD would be faster than our…

Today I performed a coating of n-Dodecyl β-D-Maltoside (“DDM”) on PDMS in an attempt to block protein adsorption (reference). The reference describes two different procedures, one in which the protein being incubated on the PDMS surface is in solution with DDM, and one in which the DDM coating is performed before the sample is washed…

Last fall we decided to try to integrate a filter immediately upstream of Vincent Tabard-Cossa’s nanopore DNA sensors. The idea is to assemble back-to-back a NPN membrane a standard nitride membrane used in Vincent’s nanopore fabrication system an oxide spacer. Currently the lifetime of these sensors/sequencers is limited by clogging events. The purpose of our…

  Here is a graphical summary of the separation experiments performed the week of November 6th. Raw excel data is spread over 5 files: 11.4.14 qdot separations (10^13, 10^12, 10^11 Forward separations) 11.5.14 high conc reverse and forward (10^14 dots and IgG combination forward and reverse separations) 11.10.14 (retentate numbers for all separations) summarized qDot data (summary and graphs)…