Temp-controlled 'nanopores' may allow detailed blood analysis

To follow up my last post on air-blood barrier TEER results, I wanted to show some Live/Dead images of these samples.  These are stained after the 14 day experiment. In the following image, you can see normal bEnd morphology on PET and pnc-Si.  Only the cells over the pnc-Si slit are visible because these cells…

We are happy to be back this summer. Anthony has been working on setting up a system that mimics Kilean’s dialysis devices. Our approach is different in two important ways. First, we are using PETLs, instead of PDMS/glass.  For those who are not familiar with PETLs, here is a short summary: Commercial laminating pouches made…

Hi all, my name is John Cognetti, and I am a first-year PhD student in BME. I recently finished a rotation in the McGrath Lab. My goal was to measure changes in resistance across the membrane as the membrane becomes saturated with particles. Initially, the plan was to use Tejas’ trans-endothelial electrical resistance (TEER) setup…

This post is related to the HIV drug screening project that Carrie has been working on.  To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes from smaller protein monomers/multimers.  Small molecules that break down the HMM form of this protein…

After discussions with the gentleman in charge of surface functionalization at INT last week, Jim suggested that I attempt to pretreat our membranes with 0.1% SDS before a filtration.  The hope being that some surfactant left on the surface of the membrane would be enough to facilitate water passage through the membrane without wetting the…