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Journal Club- Characterization of ultrafiltration membranes fouled by quantum dots by confocal laser scanning microscopy
Here’s the paper we’re discussing this week: Wu(Moulin)JMS14 The authors motivate their work by claiming that ultrafiltration membranes can be used to keep “engineered nanomaterials” (ENM) out of our waters. They use confocal laser scanning microscopy to locate the position of fluorescent CdTe quantum dots (QD) on/inside an Ultracel membrane. (Made by Millipore using regenerated…
Terminology
There has been some confusion lately with what we should name the compartments in diffusion. Here’s what I’ve come up with: Top/Compartment with initial sample: Retentate Bottom/Compartment to which things diffuse: Dialysate The original word we were using for dialysate, filtrate, implies an active process and was criticized by scientists at NAMS. While dialysate implies…
Make Sure to Filter Salts Before Passing Them Through Sepcons
Previously, I had gotten some inexplicable results showing that increasing salt concentration decreases the flow through a membrane. This makes no sense (and goes against our previous data) because we would expect electrostatic forces to counteract the flow, an effect called counterelectroosmosis by Zydney: “The situation in the presence of a net fluid flow through…
Kidney Health Initiative Roundtable: Roadmap to Advanced RRT
(This post is not finished, but my flight keeps getting delayed in 20 minute increments, so better to publish it unfinished than to not get it up before NRG in case I’m not able to make it. Please forgive typos etc.) I attended a meeting organized by the Kidney Health Initiative, a collaboration between the…
Nair, R. R., et al. (2012). “Unimpeded permeation of water through helium-leak-tight graphene-based membranes.” Science 335(6067): 442-444.
ByJimNair(Geim)Science12 Supplement Laminates of GO from 0.1 to 10 um thick on copper foil with an etched hole of 1 cm. Casually mentions >1.5 psi applied to materials < 1 um thick over 1 cm diameter hole. This seems to suggests a strong burst pressure but with no real data or experimental details, this is…
Preliminary Test of IgG Binding to Protein A Beads
Last week, Barrett and I made up the three buffers needed to perform this test: Dissociating buffer: 0.1 M glycine-HCl, pH 3.5 1 M Tris Binding buffer: 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.5 In addition, I made a solution of protein A beads that consists of 0.0166 g of beads in 50…