Nanopores May Eliminate Current Evaluation Processes Used for Detecting Epigenetics-Related Diseases

Before Anant left for India, we were doing cell adhesion studies with pnc-Si transwells and getting some weird morphologies.  Check it out here, here, here, here, here. This is in contrast with the 2D studies that Anant did for the cell culture paper in which cell adhesion looked good.  The main difference is the transwell…

I performed a discoloration experiment with w639 in EGM + 10% FBS.  I put 2 blank chips in the Sepcons membrane side down.  I then placed one Sepcon in a well with EGM alone.  The other Sepcon was placed in a well with EGM but also with another chip (labelled as Sepcon with chip, chip…

Used Thermofisher SiteClick qdot antibody labeling kit 625.  Began with 4.0mg/mL IgG and conjugated it with an unknown amount of qDots, methods provided by the SiteClick kit. The final stage in the manual [1] is an optional cleaning of the qDots to remove any unconjugated and free-floating qDots from the mixture using the nanosep omega…

In the last month, we’ve run several sets of 15 nm pnc-Si wafers over a range of RTP temperatures.  Below is a chart that illustrates the current pore size control we have by tuning annealing temp. The 900 C, 950 C, and 1000 C samples were from wafers SC 231, SC 314, and SC 316,…

Last week, I reported on the the morphology of pnc-Si deposited with different substrate biases. We hypothesized that the reason for this was due to the different initial film stress of the as-deposited a-Si. Unfortunately, the pressure-displacement method does not allow us to measure amorphous films since it requires the membrane to be nearly flat…