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Partial Etch RIE Blows out NPN Pores
As the title states, this post is about shifting an arbitrary pore distribution in an NPN layer. Why would we want to do this? We believe that the underlying pore distribution and shape influences the properties of many of our devices (my SERS sensor work included). Jim has coined the term ‘bowl-with-a-hole’ to describe the…
TEER of 1st 2 cocultures
My previous 2 posts on co-cultures presented images but I also tracked the TEER in these experiments. This post shows that data. The top 2 graphs are from the 1st co-culture experiment – the bottom graph is from the second experiment (where my PET controls died), as % Day 0: On PET, the coculture TEER…
More Burst Pressure Testing: Front to Back
My original post reported burst pressures on rectangular windows with the chips trench side down in the fixture (pressure from below). These values are low compared to square window pressures. My pressures ranged from 2 psi to 12 psi while square windows achieved 10 psi to 50 psi (Sarah Wayson’s post on square windows). So I did a limited set…
Microfluidic Permeability Assay for In Situ Monitoring of Endothelial Barrier Properties
ByAlecIntroduction As part of my thesis, I have been working on developing an assay for measuring vascular barrier properties within our microfluidic devices. Traditionally, small molecule permeability measurements are performed on cells plated in a Transwell cup. The basic protocol for these assays is initiated by introducing FITC-dextran in cell culture media into the apical…
Wafer 415-T
Wafer 415-T has been delivered to the lab. It was processed with TEOS and annealed at 950 C. There are a few usable samples, especially along the edge of the wafer. Pinhole density increases as you move towards the center. The uniformity across the wafer is good. From left to right, top to bottom: (0,1)…
Transwells interfere with Tecan measurements
This week, Christine and I have been trying to figure out if we can measure transport in the Tecan with the transwell inserts remaining within the 24-well plates. Here is a schematic of our idea (with sodium fluorescein, Na-F, as the model fluorescent molecule for transport studies): The left image is a side view, the…