Here are images of actin filaments on Formvar coated 200 mesh nickel grid. Actin filaments were polymerized exactly as before. TEM sample preparation procedure is: 1. add actin solution on Formvar grid and wait 90 sec 2. remove liquid, add same volume of 2% UA and wait 90 sec 3. remove liquid and load on…
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C). Discoloration, pH, and the bead assay were measured initially and every 24 hours. Deionized water…
On Friday(which was a great day for science). I ran a series of 5 passes of the bioconjugated gold nanoparticles through 30nm cutoff Gen 3 Sepcons. If you’ll remember, the Barcikowski lab wants the free ligand separated out from the complexes. To indirectly get the relative concentrations of gold and ligand, we measured the absorbance…
See Microfluidic Mold Making for instructions. I have assembled 3 more Barrett Chamber/ SC114 pnc-Si microfluidic devices this week. Currently, there are 2 Barrett chambers remaining and the mold is missing. The mask to make the mold still exists, however, there was a lack of N2 gas for processing (spin-coater requisite), so Henry and I…
This post has been a long time coming, but I have finally (essentially) finished all the work for Aim 1 of my thesis. (Credit to Dan for helping with part of it and getting a thesis along the way, see his post here: https://trace-bmps.org/critical-flux-of-ultrathin-silicon-membranes-in-tangential-flow-filtration-of-protein-solutions/) Up until this point, I have been attempting to validate my…
As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels. Here is the latest design: For these experiments we continued to use relatively low…
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Seems 187 was a bad choice for these experiments ( see Maryna’s post). Clearly transport through the same membrane can be possible by diffusion (Jess’ diffusion experiments with 187 worked) and impossible by convection.
This failure also illustrates the usefulness of posting data shortly after it comes in. I don’t know when Maryna had this data, but if it could have been posted before Thursday we might have picked another membrane.
Can these experiments only be done in small sets? It would be useful if 2-4 samples from each new wafer could be run to test flow rate.
If the EQ experiments are really cumbersome, we need to find a way to make them easier, or they will provide very little near-term feedback. What is the bottleneck?