Yesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room temperature). Once the PDMS was cured Henry used the UV Ozone system to again try…
One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was an effect of overseeding or if cells were migrating to the freestanding area. To this…
Quick review of “Achieving more frequent and longer dialysis for the majority: wearable dialysis and implantable artificial kidney devices” by Fissell, Roy, and Davenport see Fissell(Roy)_2013_International_Kidney.pdf in the hemodialysis section of the library. First a little background: Two type of wearable devices: Peritoneal Dialysis (PD) and Hemodialysis (HD) In PD, dialysate is pumped into and…
The goal of this rotation was to create a device to test the electro-osmotic properties of the membrane bound silicon chips. To perform this task, it was necessary to attach two glass pipettes onto both sides of a chip. The method required that the protocol be repeatable and easy to perform. Although it appears to…
This post includes some optical and SEM micrographs of a SiO2 membrane patterned by nansosphere lithography using 300 nm polystyrene beads. An SU-8 microgrid was deposited on top and the membrane was released by dissolving a bottom layer of ZnO. After the release, the membrane is rinsed in water and transferred to a clean substrate….
Last week, JP and I resurrected using TEOS as the backside pattern and experimented with using a thermal oxide as the first protective oxide layer (instead of a sputtered oxide). The idea was to see how the reduced stress in the pattern (TEOS) would affect film morphology. The thermal oxide on the frontside was an…
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Seems 187 was a bad choice for these experiments ( see Maryna’s post). Clearly transport through the same membrane can be possible by diffusion (Jess’ diffusion experiments with 187 worked) and impossible by convection.
This failure also illustrates the usefulness of posting data shortly after it comes in. I don’t know when Maryna had this data, but if it could have been posted before Thursday we might have picked another membrane.
Can these experiments only be done in small sets? It would be useful if 2-4 samples from each new wafer could be run to test flow rate.
If the EQ experiments are really cumbersome, we need to find a way to make them easier, or they will provide very little near-term feedback. What is the bottleneck?