WHERE: URMC ** Room # 4-7545 ** WHEN: Tuesday April 16th & Wednesday the 17th. TIME: 9:00-4:00 P.M. Please drop off your pipettors early as we expect a good turnout. Pipettors will be done on a first come, first served basis. Thank you!
Since the new year there have been difficulties with charging the glass plate and the charge not being enough to capture the membrane. Synthetic silk was tested to see if the glass charged better but no difference was seen between the silk and a crew wipe. When materials were first being tested to use during…
INTRODUCTION The blood-brain barrier (BBB) is a highly selective endothelial barrier that serves a crucial role in maintaining tissue homeostasis in the brain. Many current models, however, suffer from low TEER and permeability values far greater than physiological levels. Recently, human induced pluripotent stem cells (hiPSC) have been differentiated into BMECS with very high TEER…
Transferred from other blog… Wafers 398 and 418 were recently tested for burst pressure. Wafer 398 had a burst pressure range of 4.28-8.24 psi, with the great majority of intact membranes on the wafer having no or one tiny pinhole. When removing samples from this wafer more shards than usual were created, with some fragments…
To identify how much of an effect the membrane has on particular simulations, we originally came up with the idea of using a phenomenological effective diffusion to view the membrane impact (see this post). Here is the most up to date version of the figure, which was slotted for use in the simulated diffusion paper:…
Goal: study HUVEC migration on different porous substrates. HP = high porosity 0.5 μm SiO2 membrane LP = low porosity 0.5 μm SiO2 membrane NP = non-porous SiO2 membrane TCP = tissue culture polystyrene (~3 GPa [ref 1, 2]) P184 = sylgard 184 (a commonly used PDMS; Young’s modulus = 1.74-1.84 MPa [ref 3]) P527 = sylgard 527…
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Seems 187 was a bad choice for these experiments ( see Maryna’s post). Clearly transport through the same membrane can be possible by diffusion (Jess’ diffusion experiments with 187 worked) and impossible by convection.
This failure also illustrates the usefulness of posting data shortly after it comes in. I don’t know when Maryna had this data, but if it could have been posted before Thursday we might have picked another membrane.
Can these experiments only be done in small sets? It would be useful if 2-4 samples from each new wafer could be run to test flow rate.
If the EQ experiments are really cumbersome, we need to find a way to make them easier, or they will provide very little near-term feedback. What is the bottleneck?